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The Glucagon-Like Peptide 2 ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab222863) kit is designed for detection of GLP-2 in plasma, serum, and cell culture samples.
The kit has been validated using rat, human, swine, and monkey samples. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures GLP-2 in approximately 4 hours. A polyclonal antibody specific for GLP-2 has been pre-coated onto a 96-well microplate with removable strips. GLP-2 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for GLP-2, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Glucagon-like peptide 2 (GLP-2) is a thirty-three amino acid peptide that is secreted by intestinal endocrine cells alongside GLP-1. GLP-2 and GLP-1 are co-secreted from enteroendocrine L-cells located in distal intestine in response to enteral nutrient ingestion, particularly fats and carbohydrates. GLP-2 secretion is mediated by direct nutrient stimulation of the L-cells and indirect action from enteroendocrine and neural inputs. GLP-2 acts to enhance nutrient absorption by inhibiting gastric motility and secretion and by stimulating nutrient transport.
|Components||1 x 96 tests|
|Anti-GLP-2 coated Microplate (12 x 8 wells)||1 unit|
|GLP-2 Standard||1 vial|
|Biotinylated GLP-2||1 x 120µl|
|10X Diluent M Concentrate||1 x 20ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|Chromogen Substrate||1 x 8ml|
|Stop Solution||1 x 12ml|
|Sealing Tapes||3 units|
Our Abpromise guarantee covers the use of ab222863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab222863 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"