Overview

  • Product name
    Glucagon-Like Peptide 2 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Plasma 1 20 6.6%
    Plasma 2 20 4.1%
    Plasma 3 20 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 1 20 9.8%
    Plasma 2 20 8.8%
    Plasma 3 20 8.7%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.15 ng/ml
  • Range
    0.281 ng/ml - 72 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat, Human, Pig, Monkey
    Predicted to work with: Mouse, DogDoes not react with: Rabbit, Cow
  • Product overview

    The Glucagon-Like Peptide 2 ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab222863) kit is designed for detection of GLP-2 in plasma, serum, and cell culture samples.


    The kit has been validated using rat, human, swine, and monkey samples. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures GLP-2 in approximately 4 hours. A polyclonal antibody specific for GLP-2 has been pre-coated onto a 96-well microplate with removable strips. GLP-2 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for GLP-2, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Notes

    Glucagon-like peptide 2 (GLP-2) is a thirty-three amino acid peptide that is secreted by intestinal endocrine cells alongside GLP-1. GLP-2 and GLP-1 are co-secreted from enteroendocrine L-cells located in distal intestine in response to enteral nutrient ingestion, particularly fats and carbohydrates. GLP-2 secretion is mediated by direct nutrient stimulation of the L-cells and indirect action from enteroendocrine and neural inputs. GLP-2 acts to enhance nutrient absorption by inhibiting gastric motility and secretion and by stimulating nutrient transport.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab222863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of Glucagon-Like Peptide 2 standard curve in 1X Diluent M. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab222863 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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