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Immunocytochemistry/ Immunofluorescence - Glucocorticoid Receptor alpha antibody - ChIP Grade (ab3580)
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Product Name
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Glucocorticoid Receptor alpha antibody - ChIP Grade
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Glucocorticoid Receptor alpha - ChIP Grade
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Immunogen
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Synthetic peptide: CEIITNQIPKYSNGNIKK, corresponding to amino acids 755-771 of Human Glucocorticoid Receptor alpha
(Peptide available as ab39764.)
BLAST 'CEIITNQIPKYSNGNIKK' with  or 
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Reacts with
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Hu, Ms, Rat
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Specificity
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Detects GR alpha from human, mouse and rat tissues.
This antibody does not detect GR beta.
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Tested applications
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ChIP, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 4 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Corticoid
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Corticoid
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the Glucocorticoid Receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR.
The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform translocates from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR alpha has a greater affinity for GR response elements (GREs) than GR beta only when in the ligand bound state.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Glucocorticoid Receptor alpha antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Glucocorticoid Receptor alpha antibody - ChIP Grade images, references, products related to ab3580 and other tools.
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Glucocorticoid Receptor alpha antibody - ChIP Grade images:
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 Immunocytochemistry/ Immunofluorescence - Glucocorticoid Receptor alpha antibody - ChIP Grade (ab3580)
ICC/IF image of ab3580 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3580, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Glucocorticoid Receptor alpha antibody - ChIP Grade (ab3580)
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This product has been used in: (two most recent references)
Piotrowska H & Jagodzinski PP Glucocorticoid receptor alpha and beta variant expression is associated with ASF/SF2 splicing factor upregulation in HT-29 colon cancer and MCF-7 breast carcinoma cells. Arch Med Res 40:156-62 (2009). WB; Human. PubMed: 19427965
Ichijo T et al. The Smad6-histone deacetylase 3 complex silences the transcriptional activity of the glucocorticoid receptor: potential clinical implications. J Biol Chem 280:42067-77 (2005). PubMed: 16249187
See all 3 publication references for this product.
If you publish research using ab3580 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Glucocorticoid Receptor alpha
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