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Product name
Anti-Glucocorticoid Receptor antibody - ChIP Grade
See all Glucocorticoid Receptor products (17) ...
Description
Rabbit polyclonal to Glucocorticoid Receptor - ChIP Grade
Tested applications
ChIP, Gel supershift assays, IP, WB, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: CKPLILPDTKPKIKD, corresponding to amino acids 245-259 of Human Glucocorticoid Receptor.
CKPLILPDTKPKIKD
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Corticoid
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Corticoid
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. See Lerner et al., 2003.
GSA: Use at an assay dependent dilution.
IP: Use a concentration of 5 µg/ml.
WB: Use a concentration of 5 µg/ml. Predicted molecular weight: 97 kDa.Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate.
IHC-Fr: Use a concentration of 1 µg/ml. Staining of GR in rat hippocampus results in staining primarily in the nucleus.
ICC/IF: 1/40. PubMed: 19202080
Target
Function
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
Tissue specificity
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
Involvement in disease
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Increased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
Target information above from: UniProt accessionP04150
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
Database links
- Entrez Gene: 2908 Human
- Entrez Gene: 14815 Mouse
- Entrez Gene: 24413 Rat
- Omim: 138040 Human
- SwissProt: P04150 Human
- SwissProt: P06537 Mouse
- SwissProt: P06536 Rat
- Unigene: 122926 Human
see all
References for Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)
This product has been referenced in:
- Ritter HD et al. The Unliganded Glucocorticoid Receptor Positively Regulates the Tumor Suppressor Gene BRCA1 through GABP Beta. Mol Cancer Res 10:558-69 (2012). WB; Human.Read more (PubMed: 22328717) »
- O'Brien KB et al. CARM1 is required for proper control of proliferation and differentiation of pulmonary epithelial cells. Development 137:2147-56 (2010). ChIP; Mouse.Read more (PubMed: 20530543) »
See all 10 publications for this product
Publishing research using ab3579? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"