Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Nucleus >> Other
Overview
Product name
Anti-Glucocorticoid receptor-interacting protein 1 antibody [GRP01]
See all KAT13C / NCOA2 products (11) ...
Description
Mouse monoclonal [GRP01] to Glucocorticoid receptor-interacting protein 1
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
The steroid hormones, thyroid hormone, vitamin D and retinoic acid function by activating specific nuclear receptor proteins found inside of target cells. The activated receptors bind to and activate specific genes; and the products of these activated genes change cellular activities in specific ways thus constituting the biological response to the hormone. After binding to target genes, the activated nuclear receptors require the assistance of proteins called transcriptional coactivators in order to activate the target genes. GRIP 1 is a novel protein that serves as a transcriptional coactivator for steroid and nuclear hormone receptors.
Clonality
Monoclonal
Clone number
GRP01
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Neuroscience >> Neurology process >> Neural Signal Transduction
Cell Biology >> Apoptosis >> Nucleus >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
WB
Application notes
Immunoprecipitation (with Protein G): use at a concentration of 2µg / mg .
Western Blotting: use at a concentration of 1 - 2µg / ml for 2hrs at RT.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a band of ~ 160 kDa, which corresponds to the predicted molecular weight of GRIP 1.
Target
Relevance
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is KAT13C / nuclear receptor coactivator 2 (NCOA2), also termed Glucocorticoid receptor-interacting protein 1 (GRIP1).
Cellular localization
Nuclear
Alternative names
- Glucocorticoid receptor interacting protein 1 antibody
- Glucocorticoid Receptor Interacting Protein antibody
- GRIP 1 antibody
see all
Database links
- Entrez Gene: 10499 Human
- Omim: 601993 Human
- SwissProt: Q15596 Human
- Unigene: 446678 Human
References for Anti-Glucocorticoid receptor-interacting protein 1 antibody [GRP01] (ab3248)
This product has been referenced in:
- Cvoro Aet al. Selective estrogen receptor-beta agonists repress transcription of proinflammatory genes. J Immunol 180:630-6 (2008). WB; Human.Read more (PubMed: 18097065) »
See 1 publication for this product
Publishing research using ab3248? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"