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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
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- Proteins and Peptides
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab76598? Please let us know so that we can cite the reference in this datasheet
ab76598 has been referenced in 1 publications.
- Wang J et al. Overexpression of G6PD is associated with poor clinical outcome in gastric cancer. Tumour Biol 33:95-101 (2012). IHC-P; Human. PubMed: 22012600
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
IHC image of Glucose 6 Phosphate staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76598, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
ICC/IF image of ab76598 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76598, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.
Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 59 kDa
Observed band size : 59 kDa
Exposure time : 30 seconds
Glucose 6 Phosphate Dehydrogenase antibody for Western blot in Human (76598)
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