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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody
See all Glucose 6 Phosphate Dehydrogenase products (15) ...
Description
Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Sheep, Cow, Chimpanzee, Baboon, Chinese Hamster
Immunogen
Synthetic peptide derived from within residues 50 - 150 of Human Glucose 6 Phosphate Dehydrogenase.
(Peptide available as ab87367.)
Positive control
This antibody gave a positive signal in RAW 264.7 and Jurkat whole cell lysates, and in human placenta and lymph node tissue lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
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Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab76598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Target
Function
Produces pentose sugars for nucleic acid synthesis and main producer of NADPH reducing power.
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm.
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in disease
Defects in G6PD are the cause of chronic non-spherocytic hemolytic anemia (CNSHA) [MIM:305900]. Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity.
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family.
Target information above from: UniProt accessionP11413
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
Cellular localisation centrosome, cytosol, internal side of plasma membrane, intracellular membrane-bounded organelle. There are 3 isoforms produced by alternative splicing.
Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Database links
- Entrez Gene: 281179 Cow
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Entrez Gene: 24377 Rat
- Entrez Gene: 443211 Sheep
- Omim: 305900 Human
- SwissProt: P11413 Human
- SwissProt: Q00612 Mouse
see all
Anti-Glucose 6 Phosphate Dehydrogenase antibody images:
Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
Lane 3 : Lymph node (Human) Tissue Lysate - adult normal tissue (ab29871)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 59 kDa
Observed band size : 59 kDa
Exposure time : 30 seconds
Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
ICC/IF image of ab76598 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76598, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
IHC image of Glucose 6 Phosphate staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76598, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598)
ab76598 has not yet been referenced specifically in any publications.
Publishing research using ab76598? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"