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Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
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Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
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Product Name 

Glucose 6 Phosphate Dehydrogenase antibody

 

See all Glucose 6 Phosphate Dehydrogenase antibodies (12)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase

Immunogen 

Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Glucose-6-Phosphate Dehydrogenase encoded in part by exon 6 and in part by exon 7 (LocusLink ID 2539).

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, IHC-Fr, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-Fr: 1/500.
IP: Use at 2µg/mg of lysate.
WB: 1/1000 - 1/10000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency; one benefit of having G6PD deficiency is that it confers a resistance to malaria.


 

Alternative names Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2539    (Human)
14381    (Mouse)
24377    (Rat)

Omim

    

305900    (Human)

SwissProt

    

P11413    (Human)
Q00612    (Mouse)
P05370    (Rat)

Unigene

    

461047    (Human)
11040    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Glucose 6 Phosphate Dehydrogenase antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Glucose 6 Phosphate Dehydrogenase antibody images, references, products related to ab993 and other tools.

 

Glucose 6 Phosphate Dehydrogenase antibody images:

Product image  Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
 

Product image  Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab993)

ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

References for Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Specific publication references   This product has been used in: (two most recent references)

Schafer ZT et al. Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. Nature 461:109-13 (2009). WB; Human. PubMed: 19693011

Poirrier JE et al. Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation. Proteome Sci 6:14 (2008). WB; Rat. PubMed: 18498662


See all 6 publication references for this product.


If you publish research using ab993 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Glucose 6 Phosphate Dehydrogenase

 

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Glucose 6 Phosphate Dehydrogenase antibody - related products:

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Lysates

 

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Other Glucose 6 Phosphate Dehydrogenase Antibodies

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Glucose 6 Phosphate Dehydrogenase antibody - other tools:

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