The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
1/1000 - 1/10000.
Use a concentration of 5 µg/ml.
FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
Post-translational modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Frozen sections) - Glucose 6 Phosphate Dehydrogenase antibody (ab993)This image is courtesy of an anonymous Abreview
ab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.
Western Blot Lanes 1-2 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 0.5 µg/ml Immunoprecipitation Lanes 3-4 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 3.75 µg/mg lysate.
Lane 1 : Wild-type mouse peritoneal macrophage whole cell lysate at 20 µg
Lane 2 : Glucose 6 Phosphate Dehydrogenase deficient mouse peritoneal macrophage whole cell lysate at 20 µg Lane 3 : Supernatant from wild-type mouse peritoneal macrophage whole cell lysate at 1 mg Lane 4 : Immunoprecipitate from wild-type mouse peritoneal macrophage whole cell lysate at 1 mg
References for Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
This product has been referenced in:
Yang Y et al. Metabolic reprogramming for producing energy and reducing power in fumarate hydratase null cells from hereditary leiomyomatosis renal cell carcinoma. PLoS One8:e72179 (2013).
Read more (PubMed: 23967283) »
Cheng X et al. Gestational diabetes mellitus impairs Nrf2-mediated adaptive antioxidant defenses and redox signaling in fetal endothelial cells in utero. Diabetes62:4088-97 (2013).
Read more (PubMed: 23974919) »