ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Frozen sections) - Glucose 6 Phosphate Dehydrogenase antibody (ab993)This image is courtesy of an anonymous Abreview
ab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.
Western Blot Lanes 1-2 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 0.5 µg/ml Immunoprecipitation Lanes 3-4 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 3.75 µg/mg lysate.
Lane 1 : Wild-type mouse peritoneal macrophage whole cell lysate at 20 µg
Lane 2 : Glucose 6 Phosphate Dehydrogenase deficient mouse peritoneal macrophage whole cell lysate at 20 µg Lane 3 : Supernatant from wild-type mouse peritoneal macrophage whole cell lysate at 1 mg Lane 4 : Immunoprecipitate from wild-type mouse peritoneal macrophage whole cell lysate at 1 mg
References for Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
This product has been referenced in:
Yang Y et al. Metabolic reprogramming for producing energy and reducing power in fumarate hydratase null cells from hereditary leiomyomatosis renal cell carcinoma. PLoS One8:e72179 (2013).
Read more (PubMed: 23967283) »
Cheng X et al. Gestational diabetes mellitus impairs Nrf2-mediated adaptive antioxidant defenses and redox signaling in fetal endothelial cells in utero. Diabetes62:4088-97 (2013).
Read more (PubMed: 23974919) »