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Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
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Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
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Product Name
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Glucose 6 Phosphate Dehydrogenase antibody
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See all Glucose 6 Phosphate Dehydrogenase antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
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Immunogen
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Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Glucose-6-Phosphate Dehydrogenase encoded in part by exon 6 and in part by exon 7 (LocusLink ID 2539).
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Reacts with
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-Fr: 1/500.
IP: Use at 2µg/mg of lysate.
WB: 1/1000 - 1/10000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Cardiac metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency; one benefit of having G6PD deficiency is that it confers a resistance to malaria.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Glucose 6 Phosphate Dehydrogenase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Glucose 6 Phosphate Dehydrogenase antibody images, references, products related to ab993 and other tools.
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Glucose 6 Phosphate Dehydrogenase antibody images:
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 Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
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Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Glucose 6 Phosphate Dehydrogenase antibody (ab993)
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This product has been used in: (two most recent references)
Schafer ZT et al. Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. Nature 461:109-13 (2009). WB; Human. PubMed: 19693011
Poirrier JE et al. Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation. Proteome Sci 6:14 (2008). WB; Rat. PubMed: 18498662
See all 6 publication references for this product.
If you publish research using ab993 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Glucose 6 Phosphate Dehydrogenase
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Glucose 6 Phosphate Dehydrogenase antibody - more information
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Customer reviews (feedback) regarding Glucose 6 Phosphate Dehydrogenase antibody |
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Glucose 6 Phosphate Dehydrogenase antibody - related products:
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Glucose 6 Phosphate Dehydrogenase antibody - other tools:
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Contact Abcam with a Technical Enquiry about Glucose 6 Phosphate Dehydrogenase antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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