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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody
See all Glucose 6 Phosphate Dehydrogenase products (15) ...
Description
Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
Tested applications
IHC-Fr, IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Glucose-6-Phosphate Dehydrogenase encoded in part by exon 6 and in part by exon 7 (LocusLink ID 2539).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-Fr: 1/500.
IP: Use at 2µg/mg of lysate.
WB: 1/1000 - 1/10000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Produces pentose sugars for nucleic acid synthesis and main producer of NADPH reducing power.
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm.
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in disease
Defects in G6PD are the cause of chronic non-spherocytic hemolytic anemia (CNSHA) [MIM:305900]. Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity.
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family.
Target information above from: UniProt accessionP11413
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
Cellular localisation centrosome, cytosol, internal side of plasma membrane, intracellular membrane-bounded organelle. There are 3 isoforms produced by alternative splicing.
Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Database links
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Entrez Gene: 24377 Rat
- Omim: 305900 Human
- SwissProt: P11413 Human
- SwissProt: Q00612 Mouse
- SwissProt: P05370 Rat
- Unigene: 461047 Human
see all
Anti-Glucose 6 Phosphate Dehydrogenase antibody images:
Immunocytochemistry/ Immunofluorescence - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Frozen sections) - Glucose 6 Phosphate Dehydrogenase antibody (ab993)
ab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
This product has been referenced in:
- Schafer ZTet al. Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. Nature 461:109-13 (2009). WB; Human.Read more (PubMed: 19693011) »
- Poirrier JEet al. Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation. Proteome Sci 6:14 (2008). WB; Rat.Read more (PubMed: 18498662) »
See all 6 publications for this product
Publishing research using ab993? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"