Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Overview

  • Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibodySee all Glucose 6 Phosphate Dehydrogenase primary antibodies ...
  • Description
    Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Tested applicationsIHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Glucose-6-Phosphate Dehydrogenase encoded in part by exon 6 and in part by exon 7 (LocusLink ID 2539).

Properties

Applications

Our Abpromise guarantee covers the use of ab993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/10000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
  • PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in diseaseChronic non-spherocytic hemolytic anemia
  • Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

Anti-Glucose 6 Phosphate Dehydrogenase antibody images

  • ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 0.6 µg/ml

    Lane 1 : HUVEC whole cell lysate at 15 µg
    Lane 2 : HUVEC whole cell lysate at 25 µg

    developed using the ECL technique
  • Western Blot Lanes 1-2 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 0.5 µg/ml
    Immunoprecipitation Lanes 3-4 : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 3.75 µg/mg lysate.

    Lane 1 : Wild-type mouse peritoneal macrophage whole cell lysate at 20 µg
    Lane 2 : Glucose 6 Phosphate Dehydrogenase deficient mouse peritoneal macrophage whole cell lysate at 20 µg
    Lane 3 : Supernatant from wild-type mouse peritoneal macrophage whole cell lysate at 1 mg
    Lane 4 : Immunoprecipitate from wild-type mouse peritoneal macrophage whole cell lysate at 1 mg

References for Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

This product has been referenced in:
  • Yang Y  et al. Metabolic reprogramming for producing energy and reducing power in fumarate hydratase null cells from hereditary leiomyomatosis renal cell carcinoma. PLoS One 8:e72179 (2013). WB ; Human . Read more (PubMed: 23967283) »
  • Cheng X  et al. Gestational diabetes mellitus impairs Nrf2-mediated adaptive antioxidant defenses and redox signaling in fetal endothelial cells in utero. Diabetes 62:4088-97 (2013). WB ; Human . Read more (PubMed: 23974919) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (Mouse brain)
Specification Mouse brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 02 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Mouse Tissue lysate - other (Lung cytosol)
Specification Lung cytosol
Blocking step (agent) for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jul 19 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (WBCs)
Specification WBCs
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Apr 11 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Leukocytes from bone marrow)
Specification Leukocytes from bone marrow
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 28 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Bone marrow cells)
Specification Bone marrow cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 09 2010

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat testis)
Specification Rat testis
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Nov 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Schizosaccharomyces pombe Cell lysate - other (whole cell lysate)
Loading amount 100 µg
Specification whole cell lysate
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Aug 02 2010

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 19 2010

Application Western blot
Sample Rat Tissue lysate - whole (Skin)
Loading amount 15 µg
Specification Skin
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
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Submitted Apr 24 2007

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (oocytes)
Loading amount 10 µg
Specification oocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
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Submitted Aug 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"