Overview
Product nameGlucose 6 Phosphate Dehydrogenase protein (Active)See all Glucose 6 Phosphate Dehydrogenase proteins and peptides ...
Protein descriptionRecombinant full length Human Glucose 6 Phosphate Dehydrogenase (amino acids 1-515) with an N-terminal His tag; 535 amino acids with a predicted MWt of 59 kDa, NP_001035810.1
Uniprot accessionP11413
Molecular weight61.400kDa inclusive of tags
Protein length515 amino acids
Expression hostBaculovirus infected High-5 cells
Properties
Purity> 95
% by SDS-PAGE
Purification notesab126671 was purified using conventional chromatography.
Biological activitySpecific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C
E.C. Number1.1.1.49
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferpH: 8.00
Constituents: 0.002% PMSF, 0.03% DTT, 0.32% Tris HCl, 0.06% EDTA, 20% Glycerol, 1.17% Sodium chloride
Concentration information loading...
Sequence notesMGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS
QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV
VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab126671
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent concentration. |
Application notesThis peptide can be used with studies using ab61704,ab76598,ab87230 and ab106810.
Protein info
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Alternative names
G6pdxGlucose 6 phosphate 1 dehydrogenaseGlucose 6 phosphate dehydrogenaseGlucose 6 phosphate dehydrogenase, G6PDGlucose-6-phosphate 1-dehydrogenaseMET19POS10Zwf1p
see all
FunctionProduces pentose sugars for nucleic acid synthesis and main producer of NADPH reducing power.
Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in diseaseChronic non-spherocytic hemolytic anemia (CNSHA) [MIM:305900]: Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA; class-II have an activity <10% of normal; class-III have an activity of 10% to 60% of normal; class-IV have near normal activity. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
Glucose 6 Phosphate Dehydrogenase protein (Active) images
References for Glucose 6 Phosphate Dehydrogenase protein (Active) (ab126671)
ab126671
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - Glucose 6 Phosphate Dehydrogenase protein (Active) (ab126671)15% SDS-PAGE analysis of ab126671 (3µg)