For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Does not react withMouse, Rat
ab128574 GPI Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human glucose 6 phosphate isomerase (GPI) in cell and tissue lysates. The assay employs an antibody specific for human GPI coated onto well plate strips. Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti- GPI primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Typical working ranges for cell extracts:
HeLa, Hek293, Jurkat, fibroblast: 1 – 50 µg/mL
GPI protein standard: 0.5 – 1000 ng/mL
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 15ml|
|10X GPI Detector Antibody||1 x 1ml|
|10X HRP Label||1 x 1ml|
|20X Buffer||1 x 20ml|
|Extraction Buffer||1 x 15ml|
|GPI Microplate (12 x 8 antibody coated well strips)||1 unit|
|Human GPI standard||1 x 250ng|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab128574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab128574 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"