MitoSciences (MS1072)

Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)

Overview

  • Product nameGlucose 6 Phosphate Isomerase Human ELISA Kit
    See all Glucose 6 Phosphate Isomerase kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 6%
    Overall 8 6%
  • Tests
    1 x 96 well plate
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    > 0.5 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Product overview

    ab128574 GPI Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human glucose 6 phosphate isomerase (GPI) in cell and tissue lysates. The assay employs an antibody specific for human GPI coated onto well plate strips. Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti- GPI primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Notes

    Typical working ranges for cell extracts:

    HeLa, Hek293, Jurkat, fibroblast: 1 – 50 µg/mL

    GPI protein standard: 0.5 – 1000 ng/mL

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Cellular localizationCytoplasmic and Secreted
  • Alternative names
    • AMF
    • Autocrine motility factor
    • Glucose phosphate isomerase
    • Glucose-6-phosphate isomerase
    • GNPI
    • Neuroleukin
    • NLK
    • PGI
    • PHI
    • Phosphoglucose isomerase
    • Phosphohexose isomerase
    • SA-36
    • SA36
    • Sperm antigen 36
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab128574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Glucose 6 Phosphate Isomerase Human ELISA Kit images

  • Example standard curve of serially diluted recombinant GPI in the working range of the assay.
  • Example HeLa dilution series in the assay working range.
  • Demonstration of assay specificity by measuring GPI concentration in HeLa cell lysates at 50 µg/mL. Using this ELISA kit it was determined that after DFO treatment of HeLa cells to simulate hypoxia, the GPI concentration in cells increases 2.5 fold.

Protocols

References for Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)

ab128574 has not yet been referenced specifically in any publications.

Product Wall


The Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574) has been tested using tissue extracts and cell lysates as indicated on the datasheet of the product. To my knowledge G6Pi is normally and intracellular marker, however if there is good r...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"