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MitoSciences (MS1072)

Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)

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Overview

  • Product nameGlucose 6 Phosphate Isomerase Human ELISA Kit
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 6%
  • Tests
    1 x 96 well plate
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeSandwich
  • Sensitivity
    > 0.5 ng/ml
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Product overview

    ab128574 GPI Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human glucose 6 phosphate isomerase (GPI) in cell and tissue lysates. The assay employs an antibody specific for human GPI coated onto well plate strips. Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti- GPI primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Notes

    Typical working ranges for cell extracts:

    HeLa, Hek293, Jurkat, fibroblast: 1 – 50 µg/mL

    GPI protein standard: 0.5 – 1000 ng/mL

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Cellular localizationCytoplasmic and Secreted
    • Alternative names
        AMFAutocrine motility factorGlucose phosphate isomerase
        Glucose-6-phosphate isomeraseGNPINeuroleukinNLKPGIPHIPhosphoglucose isomerasePhosphohexose isomeraseSA-36SA36Sperm antigen 36
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab128574 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Glucose 6 Phosphate Isomerase Human ELISA Kit images

      • Sandwich ELISA - Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)
        Sandwich ELISA - Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)
        Example standard curve of serially diluted recombinant GPI in the working range of the assay.
      • Sandwich ELISA - Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)
        Sandwich ELISA - Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)
        Example HeLa dilution series in the assay working range.
      • Sandwich ELISA - Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)
        Sandwich ELISA - Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)
        Demonstration of assay specificity by measuring GPI concentration in HeLa cell lysates at 50 µg/mL. Using this ELISA kit it was determined that after DFO treatment of HeLa cells to simulate hypoxia, the GPI concentration in cells increases 2.5 fold.

      Protocols

      References for Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574)

      ab128574 has not yet been referenced specifically in any publications.

      Publishing research using ab128574 ? Please let us know so that we can cite the reference in this datasheet

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      Can we use serum and cerebrospinal fluid with this kit, or it is only suitable for cell lysates?


      The Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574) has been tested using tissue extracts and cell lysates as indicated on the datasheet of the product. To my knowledge G6Pi is normally and intracellular marker, however if there is good reas...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"