Glucose 6 phosphate Assay Kit (High Sensitivity) (ab107923)
- Product nameGlucose 6 phosphate Assay Kit (High Sensitivity)See all Glucose 6 phosphate kits ...
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Sensitivity> 1 µM
- Range1 µM - 500 µM
- Assay time0h 20m
- Product overview
Abcam's Glucose 6 Phosphate Assay Kit (High Sensitivity) is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. This High Sensitivity Glucose-6-Phosphate Assay Kit is much more sensitive than the Glucose-6-Phosphate Assay Kit (ab83426) .
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Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
- Storage bufferPreservative: None
Components Identifier 100 tests Glucose 6 phosphate assay buffer WM 1 x 25ml Glucose 6 phosphate Enzyme Mix (lyophilized) Green 1 vial Glucose 6 phosphate Standard (10 µmol; lyophilized) Yellow 1 vial Glucose 6 phosphate Substrate Mix (lyophilized) Red 1 vial Probe (in DMSO) Blue 1 x 0.4ml
- Research Areas
Our Abpromise guarantee covers the use of ab107923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Glucose 6 phosphate Assay Kit (High Sensitivity) images
Glucose 6 phosphate standard curves (0 – 500 and 0 – 50 pmol range) generated using ab107923.
References for Glucose 6 phosphate Assay Kit (High Sensitivity) (ab107923)
ab107923 has not yet been referenced specifically in any publications.