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Glucose 6 phosphate Assay Kit (High Sensitivity) (ab107923)

Q&A (1)

Overview

  • Product nameGlucose 6 phosphate Assay Kit (High Sensitivity)See all Glucose 6 phosphate kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 500 µM
  • Assay time
    0h 20m
  • Product overview

    Abcam's Glucose 6 Phosphate Assay Kit (High Sensitivity) is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. This High Sensitivity Glucose-6-Phosphate Assay Kit is much more sensitive than the Glucose-6-Phosphate Assay Kit (ab83426) .
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlucose 6 phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
    • Alternative names
        G6P

    Applications

    Our Abpromise guarantee covers the use of ab107923 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Glucose 6 phosphate Assay Kit (High Sensitivity) images

    • Functional Studies - Glucose 6 phosphate Assay Kit (ab107923)
      Functional Studies - Glucose 6 phosphate Assay Kit (ab107923)
      Glucose 6 phosphate standard curves (0 – 500 and 0 – 50 pmol range) generated using ab107923.

    Protocols

    References for Glucose 6 phosphate Assay Kit (High Sensitivity) (ab107923)

    ab107923 has not yet been referenced specifically in any publications.

    Publishing research using ab107923 ? Please let us know so that we can cite the reference in this datasheet

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    Hello,
    I have a question about the protocol of G6P assay kit. I would like to measure intracellular G6P level with the kit. The protocol recommends that 5 million cells should be homogenized with 2-3 volumes of ice cold PBS. I wonder if it is okay to ...

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    Thank you for contacting us. Yes the assay buffer in the kit may be used. You may sonicate the cells being careful that they are fully lysed.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more adv...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"