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Anti-Glucose 6 phosphate isomerase antibody (ab99189)

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Overview

  • Product nameAnti-Glucose 6 phosphate isomerase antibodySee all Glucose 6 phosphate isomerase primary antibodies ...
  • Description
    Goat polyclonal to Glucose 6 phosphate isomerase
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Cynomolgus Monkey, Orangutan
  • Immunogen

    Synthetic peptide: RERMFNGEKINYT by a Cysteine residue linker, corresponding to internal sequence amino acids 81-93 of Human Glucose 6 phosphate isomerase (NP_000166.2)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHuman heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).

Target

  • FunctionBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • Involvement in diseaseDefects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Sequence similaritiesBelongs to the GPI family.
  • Post-translational
    modifications    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • Cellular localizationCytoplasm. Secreted.

    • Target information above from: UniProt accession P06744 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        AMF antibodyAurocrine motility factor antibodyAutocrine motility factor antibody
        DKFZp686C13233 antibodyEC 5.3.1.9 antibodyG6PI_HUMAN antibodyGlucose phosphate isomerase antibodyGlucose-6-phosphate isomerase antibodyGNPI antibodyGPI antibodyGpi1 antibodyHexose monophosphate isomerase antibodyHexosephosphate isomerase antibodyNeuroleukin antibodyNLK antibodyOxoisomerase antibodyPGI antibodyPHI antibodyPhosphoglucose isomerase antibodyPhosphohexomutase antibodyPhosphohexose isomerase antibodyPhosphosaccharomutase antibodySA 36 antibodySA-36 antibodySA36 antibodySperm antigen 36 antibody
      see all

    Anti-Glucose 6 phosphate isomerase antibody images

    • Western blot - Glucose 6 phosphate isomerase antibody (ab99189)
      Western blot - Glucose 6 phosphate isomerase antibody (ab99189)
      Anti-Glucose 6 phosphate isomerase antibody (ab99189) at 0.3 µg/ml + Human heart lysate at 35 µg
      developed using the ECL technique

      Predicted band size : 63 kDa

    References for Anti-Glucose 6 phosphate isomerase antibody (ab99189)

    ab99189 has not yet been referenced specifically in any publications.

    Publishing research using ab99189 ? Please let us know so that we can cite the reference in this datasheet

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    Thank you for the email. We will proceed with ab55973 as described below.


    I am very pleased to hear you would like to accept our offer and test ab55973 in pig. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forpig and include this code in the &ldq...

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    We are interested at looking at G6P isomerase in pigs. Is this the
    antibody you recommend and is there any guarantee on species
    reactivity since it wasn't listed on the page? Is there a promotion
    to verify it for you?

    Thank you very much for your interest in our glucose 6 phospate isomerase antibodies.

    We have two anti-G6P isomerase antibodies predicted to work in pig due to high immunogen sequence homology, but these haven't been tested in pig. They are ab99...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"