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Glucose 6 phosphate isomerase protein (Tagged-His Tag) (ab91923)

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Overview

  • Product nameGlucose 6 phosphate isomerase protein (Tagged-His Tag)See all Glucose 6 phosphate isomerase proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 37-297 (261 amino acids) of Human Glucose 6 phosphate isomerase with an N terminal His tag. Predicted MWt: 30 kDa; UniProt ID = P06744.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 161 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRG VEAARERMFNGEKINYTEGRA VLHVALRNRSNTPILVD GKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTIT DV INIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHI AKTLAQLNPES SLFIIASKTFTTQETITNAETAKEWFL QAAKDPSAVAKHFVALSTNTTKVKEF GIDPQNMFEFWD WVGGRYSLWSAIGLSIALHVGFDNFEQLL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91923 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AMFAurocrine motility factorAutocrine motility factor
        DKFZp686C13233EC 5.3.1.9G6PI_HUMANGlucose phosphate isomeraseGlucose-6-phosphate isomeraseGNPIGPIGpi1Hexose monophosphate isomeraseHexosephosphate isomeraseNeuroleukinNLKOxoisomerasePGIPHIPhosphoglucose isomerasePhosphohexomutasePhosphohexose isomerasePhosphosaccharomutaseSA 36SA-36SA36Sperm antigen 36
      see all
  • FunctionBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • Involvement in diseaseDefects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Sequence similaritiesBelongs to the GPI family.
  • Post-translational
    modifications    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • Cellular localizationCytoplasm. Secreted.

    • Target information above from: UniProt accession P06744 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Glucose 6 phosphate isomerase protein (Tagged-His Tag) images

    • SDS-PAGE - Glucose 6 phosphate isomerase protein (Tagged-His Tag) (ab91923)
      SDS-PAGE - Glucose 6 phosphate isomerase protein (Tagged-His Tag) (ab91923)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Glucose 6 phosphate isomerase protein (Tagged-His Tag) (ab91923)

    ab91923 has not yet been referenced specifically in any publications.

    Publishing research using ab91923 ? Please let us know so that we can cite the reference in this datasheet

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