Glucose Dehydrogenase Assay Kit (Colorimetric) (ab102532)

Overview

  • Product nameGlucose Dehydrogenase Assay Kit (Colorimetric)
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.01 mU/well
  • Assay time
    2h 00m
  • Product overview

    Abcam's Glucose Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This assay detects GDH activity as low as 0.01 mU.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceGlucose dehydrogenase (NAD+) (EC 1.1.1.118) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ = D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
  • Alternative names
    • GDH

Applications

Our Abpromise guarantee covers the use of ab102532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glucose Dehydrogenase Assay Kit (Colorimetric) images

  • Example of NADH standard curve obtained using ab102532
  • Example of Bovine Liver Sample test curves obtained using ab102532

Protocols

References for Glucose Dehydrogenase Assay Kit (Colorimetric) (ab102532)

ab102532 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"