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Glucose Oxidase Activity Assay Kit (Fluorometric) (ab219924) provides a simple method to measure glucose oxidase (GOx) activity in cell and tissue extracts, as well as physiological solutions. The assay uses our AbRed Indicator to monitor glucose oxidase activity, leading to an increase in absorbance that can be easily detected at OD575 nm.
This assay can detect activity from as low as 12.5 mU/mL glucose oxidase in solution. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.
Glucose oxidase (notatin, GOx, EC 22.214.171.124) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present.
Glucose oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, Glucose oxidase is commonly used in biosensors to detect glucose.
|Assay Buffer||1 x 50ml|
|DMSO||1 x 200µl|
|Glucose Oxidase (100 U)||1 vial|
|Horseradish Peroxidase (HRP)||1 vial|
|Red Dye (light-sensitive)||1 vial|
Our Abpromise guarantee covers the use of ab219924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Glucose Oxidase Activity Assay Kit (Colorimetric) (ab219924). Typical glucose oxidase dose response curve. Absorbance was measured on a 96-well clear bottom plate using a SpectraMax microplate reader with path check on. As low as 12.5 mU/mL glucose oxidase activity can be detected after 30 minutes incubation.
ab219924 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"