Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)

Overview

• Product nameAnti-Glucose Transporter GLUT2 antibody [5D1]See all Glucose Transporter GLUT2 primary antibodies ...
• Description
  Mouse monoclonal [5D1] to Glucose Transporter GLUT2
• Specificityab85715 recognizes Glucose Transporter GLUT2 expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the Human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3.
• Tested applicationsIHC-P, ICC, Flow Cyt, IHC-FoFr, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Human Glucose Transporter GLUT2 transfected NS0 cells.

• Positive controlLiver tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 5% Trehalose, 40% Glycerol, PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number5D1
• IsotypeIgG2a
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Developmental Biology
  • Lineage specification
  • Endoderm

  • Stem Cells
  • Lineage Markers
  • Endoderm

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

Applications

Target

• FunctionFacilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
• Tissue specificityLiver, insulin-producing beta cell, small intestine and kidney.
• Involvement in diseaseFanconi-Bickel syndrome (FBS) [MIM:227810]: Rare, well-defined clinical entity, inherited in an autosomal recessive mode and characterized by hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
• Post-translational
  modificationsN-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
• Cellular localizationMembrane.

Target information above from: UniProt accession P11168 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6514 Human
  • Omim: 138160 Human
  • SwissProt: P11168 Human
  • Unigene: 167584 Human

• Alternative names
  liver antibodyGlucose Transporter 2 antibodyGlucose Transporter GLUT2 antibody

  Glucose transporter type 2 antibodyGlucose transporter type 2 liver antibodyGLUT-2 antibodyGLUT2 antibodyGTR2_HUMAN antibodyGTT2 antibodySLC2A2 antibodySolute carrier family 2 (facilitated glucose transporter) member 2 antibodySolute carrier family 2 facilitated glucose transporter member 2 antibodySolute carrier family 2, facilitated glucose transporter member 2 antibody

  see all

Anti-Glucose Transporter GLUT2 antibody [5D1] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glucose Transporter GLUT2 antibody [5D1] (ab85715)
  ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.
• 

  Western blot - Glucose Transporter GLUT2 antibody [5D1] (ab85715)
  Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816) at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 57 kDa
  Observed band size : 57 kDa
  
  
  Exposure time : 8 minutes
• 

  Flow Cytometry-Anti-Glucose Transporter GLUT2 antibody [5D1](ab85715)
  Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  
• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)Image from Vargas N et al., PLoS One. 2011;6(8):e22364. Epub 2011 Aug 4. Fig 5.; doi:10.1371/journal.pone.0022364; August 4, 2011, PLoS ONE 6(8): e22364.
  Immunohistochemical analysis of frozen pancreatic tissue in control (left) or TAT-MafA mutant (right) neonate mice.
  
  Glucose Transporter GLUT2 was stained using ab85715 (green), at 10 mg/ml. An AlexaFluor®647-conjugated donkey anti-mouse IgG was used as the secondary antibody.