Anti-Glucose Transporter GLUT2 antibody (ab54460)

Overview

  • Product nameAnti-Glucose Transporter GLUT2 antibody
    See all Glucose Transporter GLUT2 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT2
  • SpecificityThis antibody recognizes the ~53-61 kDa GLUT2 protein. It does not recognize other GLUT isoforms.
  • Tested applicationsWB, Indirect ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide conjugated to KLH corresponding to amino acids in the cytoplasmic domain of rat Glucose Transporter GLUT2.

  • General notesFollowing initial thaw, aliquot and freeze (-20°C).

Applications

Our Abpromise guarantee covers the use of ab54460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 57 kDa.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionFacilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
  • Tissue specificityLiver, insulin-producing beta cell, small intestine and kidney.
  • Involvement in diseaseFanconi-Bickel syndrome
  • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • liver antibody
    • Glucose Transporter 2 antibody
    • Glucose Transporter GLUT2 antibody
    • Glucose transporter type 2 antibody
    • Glucose transporter type 2 liver antibody
    • Glucose transporter, liver/islet antibody
    • GLUT-2 antibody
    • GLUT2 antibody
    • GTR2_HUMAN antibody
    • GTT2 antibody
    • SLC2A2 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 2 antibody
    • Solute carrier family 2 facilitated glucose transporter member 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 2 antibody
    see all

Anti-Glucose Transporter GLUT2 antibody images

  • ICC/IF image of ab54460 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Glucose Transporter GLUT2 antibody (ab54460)

This product has been referenced in:
  • Li YG  et al. 1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice. Sci Rep 3:1377 (2013). WB ; Mouse . Read more (PubMed: 23536174) »
  • Li YG  et al. Cooperative anti-diabetic effects of deoxynojirimycin-polysaccharide by inhibiting glucose absorption and modulating glucose metabolism in streptozotocin-induced diabetic mice. PLoS One 8:e65892 (2013). WB ; Mouse . Read more (PubMed: 23755289) »

See all 3 Publications for this product

Product Wall

The ab8297 and ab85715 has been added to the order 1075844.

Thank you very muchfor your cooperation in this case.

Could you let me know which antibody you are interested in to get we have following antibodies in catalogue;

ab85715 and ab95256 are tested in ICC/IF on human cells

ab115199 and ab8297 are also tested in ICC/IF on human cells.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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Vielen Dank für Ihre Anfrage.

Leider haben wir keinen Antikörper, der allen genannten Anforderungen entspricht.

Wir führen einen Maus-monoklonalen GLUT2-Antikörper, der bislang nur für humane Proben getestet wurde (ab85715). Das I...

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Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab54460Glucose Transporter GLUT2 antibodyhas never been tested in immunohistochemistry on tissue sections (IHC-P paraffin embedded or IHC-Fr Frozen). All testedap...

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Thank you for your message. I can confirm that the immunogen is as follows on the datasheet: A synthetic peptide conjugated to KLH corresponding to amino acids in the cytoplasmic domain of rat Glucose Transporter GLUT2. This is therefore raised a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"