Anti-Glucose Transporter GLUT4 antibody (ab654)

Overview

  • Product nameAnti-Glucose Transporter GLUT4 antibodySee all Glucose Transporter GLUT4 primary antibodies ...
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT4
  • SpecificityGLUT4.
  • Tested applicationsWB, IP, ICC, Immunomicroscopy, IHC-Fr, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Armenian Hamster
  • Immunogen

    Synthetic peptide corresponding to the C-terminal 15 residues of rat Glut4 cross-linked to Keyhole limpet hemocyanin (Peptide available as ab115831.)

Properties

Applications

Our Abpromise guarantee covers the use of ab654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/2000 - 1/2500. Detects a band of approximately 45 kDa (predicted molecular weight: 54.8 kDa).Can be blocked with Rat Glucose Transporter GLUT4 peptide (ab115831). The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration. Can also can be used for Immunogold EM (purified IgG). See references for specific protocols.
IHC-Fr 1/500.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInsulin-regulated facilitative glucose transporter.
  • Tissue specificitySkeletal and cardiac muscles; brown and white fat.
  • Involvement in diseaseDiabetes mellitus, non-insulin-dependent
  • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationCell membrane. Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Target information above from: UniProt accession P14672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

Anti-Glucose Transporter GLUT4 antibody images

  • ab654 at a 1/300 dilution, staining human GLUT4 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • ab654 at 1/500 dilution staining GLUT4 in mouse muscle by immunohistochemistry (frozen sections). Sections were methanol fixed prior to blocking in 5% goat serum for 1 hour at 25°C and then incubated with ab654 for 1 hour at 25°C. A goat polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab654 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab654, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Glucose Transporter GLUT4 antibody (ab654)

This product has been referenced in:
  • Costello PM  et al. Lower Maternal Body Condition During Pregnancy Affects Skeletal Muscle Structure and Glut-4 Protein Levels But Not Glucose Tolerance in Mature Adult Sheep. Reprod Sci N/A:N/A (2013). Sheep . Read more (PubMed: 23420826) »
  • Ndisang JF & Mishra M The heme oxygenase system selectively suppresses the proinflammatory macrophage m1 phenotype and potentiates insulin signaling in spontaneously hypertensive rats. Am J Hypertens 26:1123-31 (2013). Read more (PubMed: 23757400) »

See all 31 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Human Tissue sections (Endometriosis)
Specification Endometriosis
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Brett McKinnon

Verified customer

Submitted Jun 10 2013

Hello and thank you for sending your image. We do highly recommend boiling your samples with this antibody, and this may reduce background. However, this target is phosphorylated and glycosylated which is another reason you may see two strong bands. If...

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Thank you for your new contact. My colleague is out of office today and she has asked me to look after her customers.

Currently, we do not have a different Western blot image available.The predicted molecular weight of GLUT4 is54.8 kDa, howev...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Mesenchymal stem cells)
Specification Mesenchymal stem cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Steven Johnstone

Verified customer

Submitted Sep 11 2012

Thank you for contacting us.

All the images from WB assay in the datasheet belong to Abreviews from other users. I am afraid we do not have access to the complete images.

As new images or references are brought to our attention, the...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your enquiry.

I can confirm as discussed on the telephone that ab654 Glucose Transporter GLUT4 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture superna...

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Thank you for your message.

I am sorry that this product did not work in your application. In order to arrange a refund/credit note, I would appreciate if you could confirm your order number or PO number used to purchase this product? Read More

Vielen Dank für Ihre Antwort.

Ich habe rote Kästchen um die Bande gemalt, die ich für die richtige halte. Leider sind Proteinmarker nur eine ungefähre Größenangabe und meine 48kDa sind geschätzt wie üb...

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Vielen Dank für Ihre Antwortund dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen. Frau Schulte ist heute leider nicht im Buero.

Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"