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Synthetic peptide corresponding to the C-terminal 15 residues of rat Glut4 cross-linked to Keyhole limpet hemocyanin (Peptide available as ab115831.)
Our Abpromise guarantee covers the use of ab654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/2500. Detects a band of approximately 45 kDa (predicted molecular weight: 54.8 kDa).Can be blocked with Rat Glucose Transporter GLUT4 peptide (ab115831).
The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
It is recomended to use 3% milk block.
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration. Can also can be used for Immunogold EM (purified IgG). See references for specific protocols.|
|IHC-P||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"