Overview

  • Product name
    Glucose Uptake Assay Kit (Cell-based)
    See all Glucose kits
  • Detection method
    Flow cytometry-fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Species reactivity
    Reacts with: Human
  • Product overview

    Glucose Uptake Assay Kit (Cell-based) (ab204702) is an easy-to-use, non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin and growth factors. The assay is based on a fluorescent glucose analog, which just like glucose can be taken up by cells through glucose transporters. However, this glucose analog cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to the glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. 

  • Notes

    Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolism and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate.

  • Tested applications
    Suitable for: FM, Flow Cyt, Functional Studiesmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    Analysis Buffer (50X) 1 x 1.8ml
    GluTracker Enhancer 1 x 1ml
    GluTracker Reagent (100X) 1 x 200µl
    Phloretin (100X) 1 x 75µl
  • Research areas
  • Relevance
    Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.

Applications

Our Abpromise guarantee covers the use of ab204702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Images

  • Comparison of histogram from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).

  • Images of Jurkat cells obtained using fluorescent microscope (Top: treated with phloretin; Bottom: without phloretin treatment).

  • Glucose Uptake in HeLa cells: HeLa cells showing the uptake of GluTracker Reagent in the cytoplasm. Cells were stained with GluTracker Reagent for 30 minutes and fixed. Image was taken using a fluorescent microscope with a 60X objective lens.

Protocols

References

ab204702 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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