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Glucose Uptake Assay Kit (Cell-based) (ab204702) is an easy-to-use, non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin and growth factors. The assay is based on a fluorescent glucose analog, which just like glucose can be taken up by cells through glucose transporters. However, this glucose analog cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to the glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake.
Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolism and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate.
|Analysis Buffer (50X)||1 x 1.8ml|
|GluTracker Enhancer||1 x 1ml|
|GluTracker Reagent (100X)||1 x 200µl|
|Phloretin (100X)||1 x 75µl|
Our Abpromise guarantee covers the use of ab204702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
Comparison of histogram from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).
Images of Jurkat cells obtained using fluorescent microscope (Top: treated with phloretin; Bottom: without phloretin treatment).
Glucose Uptake in HeLa cells: HeLa cells showing the uptake of GluTracker Reagent in the cytoplasm. Cells were stained with GluTracker Reagent for 30 minutes and fixed. Image was taken using a fluorescent microscope with a 60X objective lens.
ab204702 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"