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Overview

  • Product nameGlucose and Sucrose Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (OD570nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glucose (C6H12O6; FW: 180.16) and sucrose (C12H22O11; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceSucrose (C12H22O11; FW:342.3) is a disaccharide composed of glucose (C6H12O6; FW: 180.16) and fructose (C6H12O6; FW:180.16). Sucrose can be broken down by different In the human body, sucrose can be broken down by sucrase and/or invertase into its components, which are rapidly absorbed into the bloodstream. Once inside the cells, both monosaccharides will be processed and enter the glycolysis pathway to ultimately produce energy in form of ATP.

    • Applications

    Our Abpromise guarantee covers the use of ab65334 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Glucose and Sucrose Assay Kit images

    • Functional Studies - Glucose and Sucrose Assay Kit (ab65334)
      Functional Studies - Glucose and Sucrose Assay Kit (ab65334)
      Standard calibration Curve of Sucrose.

    Protocols

    References for Glucose and Sucrose Assay Kit (ab65334)

    This product has been referenced in:
    • Rodríguez D  et al. Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23418305) »

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    We are using primary stem cells and want to test glucose and sucrose levels at different time points using ab65334. Will this kit be suitable for primary stem cells? We would lyse the cells a these various time points before testing with the assay.  ...

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    Thank you for your telephone enquiry yesterday. I have gathered some information to help answer your questions: 1. I can confirm you should be able to use stem cells with this kit, however the cell number needed to obtain readings in the linear range ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"