Glucose and Sucrose Assay Kit (ab65334)
- Product nameGlucose and Sucrose Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Assay time0h 40m
- Product overview
Abcam's Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (OD570nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
Visit our FAQs page for tips and troubleshooting.
Glucose (C6H12O6; FW: 180.16) and sucrose (C12H22O11; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Glucose Enzyme Mix (lyophilized) Green 1 vial Glucose Probe (in DMSO) Red 1 x 200µl Invertase (Lyophilized) Blue 1 vial Sucrose Standard (100 nmol/ul) Yellow 1 x 100µl
- Research Areas
Our Abpromise guarantee covers the use of ab65334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Glucose and Sucrose Assay Kit (ab65334)
This product has been referenced in:
- Rodríguez D et al. Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23418305) »