Glutamate Oxidase Assay Kit (Fluorometric - Near Infrared) (ab138885)

Overview

  • Product name
    Glutamate Oxidase Assay Kit (Fluorometric - Near Infrared)
  • Sample type
    Cell Lysate
  • Assay type
    Enzyme activity
  • Sensitivity
    = 40 uU/ml
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    Glutamate Oxidase Assay Kit (Fluorometric - Near Infrared) (ab138885) provides a quick and an ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to a-ketoglutarate, NH3 and H2O2 by glutamate oxidase. The kit uses our AbRed Indicator substrate which can react with H2O2 when catalyzed by horseradish peroxidase (HRP) to generate the highly fluorescent product. The signal can be read with either a fluorescence microplate reader at Ex/Em = 530-570 nm/590 -600 nm (optimal Ex/Em = 540 nm/590 nm) or an absorbance microplate reader at 576±5 nm. With this product, we have detected as little as 40 µU/mL glutamate oxidase in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

  • Notes

    Glutamate oxidase specifically catalyzes the oxidative deamination of L-glutamate in the presence of water and oxygen with the formation of ketoglutarate, ammonia, and hydrogen peroxide. Glutamate oxidase can be used as an analytic reagent and as a basis for developing biosensors for the determination of L-glutamate, L-glutamine, ammonia, and creatinine. These biosensors can be used in clinical biochemistry for the determination of glutamate-pyruvate transaminase and glutamate-oxalacetate transaminase in biological fluids, which makes the early diagnosis of heart and liver diseases possible.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab138885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Glutamate oxidase dose response was measured on a 96- well black plate with ab138885. As low as 40 µU/mL glutamate oxidase can be detected with 60 minutes incubation time (n=3).

Protocols

References

ab138885 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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