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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-EAAT2 antibody
See all EAAT2 products (10) ...
Description
Rabbit polyclonal to EAAT2
Tested applications
IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Rat Glutamate Transporter 1 (EAAT2).
(Peptide available as ab41752.)
Positive control
ab41621 gave a positive signal in both Mouse and Rat Brain Tissue Lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurotransmitter >> Transporters >> Glutamate
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Western blot - EAAT2 antibody (ab41621)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab41621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
IHC-FoFr: Use at an assay dependent concentration.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionP43004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EAA2_HUMAN antibody
- EAAT2 antibody
- Excitatory amino acid transporter 2 antibody
see all
Database links
- Entrez Gene: 6506 Human
- Entrez Gene: 20511 Mouse
- Entrez Gene: 29482 Rat
- Omim: 600300 Human
- SwissProt: P43004 Human
- SwissProt: P43006 Mouse
- SwissProt: P31596 Rat
- Unigene: 502338 Human
see all
Anti-EAAT2 antibody images:
Western blot - EAAT2 antibody (ab41621)
All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml
Lane 1 : Brain (Mouse) Mouse Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 62 kDa
Observed band size : 62 kDa
References for Anti-EAAT2 antibody (ab41621)
This product has been referenced in:
- Reissner KJet al. Use of vivo-morpholinos for control of protein expression in the adult rat brain. J Neurosci Methods : (2011). WB; Rat.Read more (PubMed: 22027492) »
- Tizon Bet al. Cystatin C protects neuronal cells from amyloid-beta-induced toxicity. J Alzheimers Dis 19:885-94 (2010).Read more (PubMed: 20157244) »
See all 2 publications for this product
Publishing research using ab41621? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"