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Anti-EAAT2 antibody (ab41621)

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Overview

Product name

Anti-EAAT2 antibody
See all EAAT2 products (10) ...

Description

Rabbit polyclonal to EAAT2

Tested applications

IHC-FoFr, WBmore details

Cross reactivity

Reacts with

Mouse, Rat

Predicted to work with

Human

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Rat Glutamate Transporter 1 (EAAT2).

(Peptide available as ab41752.)

Positive control

ab41621 gave a positive signal in both Mouse and Rat Brain Tissue Lysates

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - EAAT2 antibody (ab41621)Western blot - EAAT2 antibody (ab41621) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab41621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-FoFr: Use at an assay dependent concentration.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.

Post-translational
modifications

Glycosylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionP43004 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EAA2_HUMAN antibody
  • EAAT2 antibody
  • Excitatory amino acid transporter 2 antibody
  • Excitotoxic amino acid transporter 2 antibody
  • Glial high affinity glutamate transporter antibody
  • GLT 1 antibody
  • GLT1 antibody
  • Glutamate aspartate transporter II antibody
  • Glutamate/aspartate transporter II antibody
  • SLC1A2 antibody
  • Sodium dependent glutamate aspartate transporter 2 antibody
  • Sodium-dependent glutamate/aspartate transporter 2 antibody
  • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
  • Solute carrier family 1 member 2 antibody
see all

Anti-EAAT2 antibody images:

  Western blot - EAAT2 antibody (ab41621)

Western blot - EAAT2 antibody (ab41621)

All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml

Lane 1 : Brain (Mouse) Mouse Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 62 kDa
Observed band size : 62 kDa

References for Anti-EAAT2 antibody (ab41621)

This product has been referenced in:

  • Reissner KJet al. Use of vivo-morpholinos for control of protein expression in the adult rat brain. J Neurosci Methods : (2011). WB; Rat.Read more (PubMed: 22027492) »
  • Tizon Bet al. Cystatin C protects neuronal cells from amyloid-beta-induced toxicity. J Alzheimers Dis 19:885-94 (2010).Read more (PubMed: 20157244) »

See all 2 publications for this product

Publishing research using ab41621? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"