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Western blot - EAAT2 antibody (ab41621)
(enlarge)
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Product Name
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EAAT2 antibody
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See all EAAT2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EAAT2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Rat Glutamate Transporter 1 (EAAT2). (Peptide available as ab41752.)
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Tested applications
(see key)
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IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-FoFr: Use at an assay dependent concentration.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab41621 gave a positive signal in both Mouse and Rat Brain Tissue Lysates
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Cellular localization
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Membrane; Multi-pass membrane protein
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Research areas
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Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurotransmitter >> Transporters >> Glutamate
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Relevance
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EAAT2 is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 0.70 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAAT2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EAAT2 antibody images, references, products related to ab41621 and other tools.
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EAAT2 antibody images:
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 Western blot - EAAT2 antibody (ab41621)
All lanes : EAAT2 antibody (ab41621) at 1 µg/ml
Lane 1 : Brain (Mouse) Mouse Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 62 kDa
Observed band size : 62 kDa
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Search PubMed (MEDLINE) for references to EAAT2
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EAAT2 antibody - more information
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Customer reviews (feedback) regarding EAAT2 antibody |
Customer FAQs regarding EAAT2 antibody |
Protocols for EAAT2 antibody |
Price and availability of products related to EAAT2 antibody |
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EAAT2 antibody - related products:
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EAAT2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EAAT2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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