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Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)

Q&A (1)

Overview

  • Product nameGlutamic Oxaloacetic Transaminase Activity Assay KitSee all Glutamic Oxaloacetic Transaminase kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    10 mU/well
  • Product overview

    In Abcam's Glutamic Oxaloacetic Transaminase Activity Assay Kit an amino group is transferred of from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of GOT with a detection limit of 10 mU per well.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutamate-oxaloacetate transaminase (GOT), also known as Aspartate aminotransferase (AST) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, GOT has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Glutamate Standard (0.1M) Yellow 1 x 0.1ml
    Glutamic Oxaloacetic Transaminase Buffer WM 1 x 25ml
    Glutamic Oxaloacetic Transaminase Developer Red 1 vial
    Glutamic Oxaloacetic Transaminase Enzyme Mix Green 1 vial
    Glutamic Oxaloacetic Transaminase Positive Control Blue 1 vial
    Glutamic Oxaloacetic Transaminase Substrate Orange 1 vial
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab105135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies FuncS

Glutamic Oxaloacetic Transaminase Activity Assay Kit images

  • Functional Studies - Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)
    Functional Studies - Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)
    Example of Standard Curve and Positive control detection using ab105135.

Protocols

References for Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)

ab105135 has not yet been referenced specifically in any publications.

Publishing research using ab105135 ? Please let us know so that we can cite the reference in this datasheet

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Submit a Question for ab105135

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I am interested in the ab105135 kit. The protocol states that formation of Glutamate is measured by by a colorless probe emitting light at 450 nm. Can you tell me the nature of this probe (chemical structure and mechanism) How exactly does this kit work?

The identity of all the kit components is proprietary information. The mechanism of the reaction is exactly as mentioned on the datasheet. The substrate is a sequence the AST and the other enzymes in the provided mix will act upon. The probe will interact...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"