Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)
- Product nameGlutamic Oxaloacetic Transaminase Activity Assay KitSee all Aspartate Aminotransferase kits ...
- Detection methodColorimetric
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeEnzyme activity
- Sensitivity10 mU/well
- Assay durationMultiple steps standard assay
- Product overview
In Abcam's Glutamic Oxaloacetic Transaminase Activity Assay Kit an amino group is transferred of from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of GOT with a detection limit of 10 mU per well.
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Glutamate-oxaloacetate transaminase (GOT), also known as Aspartate aminotransferase (AST) is a transaminase (EC 188.8.131.52) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, GOT has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Glutamate Standard (0.1M) Yellow 1 x 0.1ml Glutamic Oxaloacetic Transaminase Buffer WM 1 x 25ml Glutamic Oxaloacetic Transaminase Developer Red 1 vial Glutamic Oxaloacetic Transaminase Enzyme Mix Green 1 vial Glutamic Oxaloacetic Transaminase Positive Control Blue 1 vial Glutamic Oxaloacetic Transaminase Substrate Orange 1 vial
- Research Areas
Our Abpromise guarantee covers the use of ab105135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Glutamic Oxaloacetic Transaminase Activity Assay Kit images
Example of Standard Curve and Positive control detection using ab105135.
References for Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)
ab105135 has not yet been referenced specifically in any publications.