Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)

Overview

  • Product nameGlutamic Oxaloacetic Transaminase Activity Assay Kit
    See all Aspartate Aminotransferase kits
  • Detection methodColorimetric
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    10 mU/well
  • Assay durationMultiple steps standard assay
  • Product overview

    In Abcam's Glutamic Oxaloacetic Transaminase Activity Assay Kit an amino group is transferred of from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of GOT with a detection limit of 10 mU per well.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutamate-oxaloacetate transaminase (GOT), also known as Aspartate aminotransferase (AST) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, GOT has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).

  • Tested applicationsFunctional Studiesmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Glutamate Standard (0.1M) Yellow 1 x 0.1ml
    Glutamic Oxaloacetic Transaminase Buffer WM 1 x 25ml
    Glutamic Oxaloacetic Transaminase Developer Red 1 vial
    Glutamic Oxaloacetic Transaminase Enzyme Mix Green 1 vial
    Glutamic Oxaloacetic Transaminase Positive Control Blue 1 vial
    Glutamic Oxaloacetic Transaminase Substrate Orange 1 vial
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab105135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent dilution.

    Glutamic Oxaloacetic Transaminase Activity Assay Kit images

    • GOT activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=60 min).

    • GOT activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1=2 min; T2=60 min) with background signals subtracted.

    • GOT activity measured in cell culture medium and control medium, background signal subtracted. T1=2 min; T2=60 min.

    • GOT activity measured in cell lysates (T1=2 min; T2=60 min; duplicates (+/- SD), background signal subtracted).

    • Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.

    • Example of Standard Curve and Positive control detection using ab105135.

    Protocols

    References for Glutamic Oxaloacetic Transaminase Activity Assay Kit (ab105135)

    ab105135 has not yet been referenced specifically in any publications.

    Product Wall



    It is recommended to measure standard curve at both times 10 mins and 60 mins. Then use the difference in Abs at 60min - Abs at 10min to plot the Std curve (OD on Y axis versus Glutamate nmol on the X axis).

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    The identity of all the kit components is proprietary information. The mechanism of the reaction is exactly as mentioned on the datasheet. The substrate is a sequence the AST and the other enzymes in the provided mix will act upon. The probe will inter...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"