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Glutathione Detection Assay Kit (Fluorometric) (ab65322)

Q&A (2)Specific References (1)

Overview

  • Product nameGlutathione Detection Assay Kit (Fluorometric)See all Glutathione kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Assay time
    2h 00m
  • Product overview

    Abcam's Glutathione Detection Assay Kit (Fluorometric) provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex/Em = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.
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  • Notes

    Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis.
    • Alternative names
        GSHGSSG

    Applications

    Our Abpromise guarantee covers the use of ab65322 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Glutathione Detection Assay Kit (Fluorometric) images

    • Glutathione Detection Kit (Fluorometric) (ab65322)
      Glutathione Detection Kit (Fluorometric) (ab65322)
      Glutathione pool measured in THP-1 macrophages:

      uninfected cells;

      WT: infected with M.tuberculosis wild type;

      KO: infected with M.tuberculosis OppD knock-out;

      COM: infected with M.tuberculosis OppD knock-out complemented with OppDA gene.

      106 cells were infected and lysed by treating them with 100µl of ice cold lysis buffer. Cell lysate was diluted and mixed as described in the kit protocol. After 30 min incubation at 37C, fluorescence was measured at Ex=380nm/ Em=460nm. Results represent the means of ± S.D. of three determinations.
      Image obtained from Dasgupta A. et al; PLoS One; 2010 Aug 17; 5(8): e12225.
    • Functional Studies - Glutathione Detection Kit (Fluorometric) (ab65322)
      Functional Studies - Glutathione Detection Kit (Fluorometric) (ab65322)
      Glutathione assays were performed using various amounts of Glutathione as indicated. Results were analyzed according to the kit instructions.

    Protocols

    References for Glutathione Detection Assay Kit (Fluorometric) (ab65322)

    This product has been referenced in:
    • Dasgupta A  et al. An oligopeptide transporter of Mycobacterium tuberculosis regulates cytokine release and apoptosis of infected macrophages. PLoS One 5: (2010). Read more (PubMed: 20808924) »

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    Submit a Question for ab65322

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    Hi,

    I am using Greiner Thincert Culture Inserts for 6-well plates and A549 cells in my exposure experiments. The goal is to expose the cells at an air/liquid interphase for a certain period and to measure cell viability, oxidative stress marker...

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    Thank you for your enquiry and interest in our products.

    MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the cells...

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    Dear technical team, Our customer would like to purchase ab65322 and wants to confirm plasma samples are availble for this kit.   Please check this for our customer.     Best regards,

    Thank you for contacting us. The Glutathione Fluorometric Detection Kit (ab65322) can be used with a number of different types of sample: Cells, tissues or liquid samples such as plasma. As long as your sample contains Glutathione which can be detected th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"