Overview

  • Product name
    Anti-Glutathione Peroxidase 3 antibody
    See all Glutathione Peroxidase 3 primary antibodies
  • Description
    Goat polyclonal to Glutathione Peroxidase 3
  • Specificity
    ab59524 may crossreact with epididymis-specific Glutathione peroxidase 5(GPX5) and olfactory epithelium-speciific Glutathione peroxidase 6 (GPX6).
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Pig
    Predicted to work with: Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-NQFGKQEPGENSE

    , corresponding to internal sequence amino acids 102-114 of Human Glutathione Peroxidase 3

  • Positive control
    • Mouse Heart, Kidney and Liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab59524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-Fr Use a concentration of 20 µg/ml.

Target

  • Function
    Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
  • Tissue specificity
    Secreted in plasma.
  • Sequence similarities
    Belongs to the glutathione peroxidase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Extracellular glutathione peroxidase antibody
    • Glutathione peroxidase 3 (plasma) antibody
    • Glutathione peroxidase 3 antibody
    • GPX 3 antibody
    • GPx P antibody
    • GPx-3 antibody
    • GPx-P antibody
    • GPX3 antibody
    • GPX3_HUMAN antibody
    • GPXP antibody
    • GSHPx 3 antibody
    • GSHPx P antibody
    • GSHPx-3 antibody
    • GSHPx-P antibody
    • Plasma glutathione peroxidase 3 antibody
    • Plasma glutathione peroxidase antibody
    see all

Images

  • ab59524 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney following microwave antigen retrieval with citrate buffer pH 3 and CY3-staining showing vesicular staining of the cytoplasm in PCT epithelial cells.

  • Anti-Glutathione Peroxidase 3 antibody (ab59524) at 1 µg/ml + Mouse Heart lysate in RIPA buffer at 35 µg

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Detected by chemiluminescence

References

This product has been referenced in:
  • Civantos E  et al. Sitagliptin ameliorates oxidative stress in experimental diabetic nephropathy by diminishing the miR-200a/Keap-1/Nrf2 antioxidant pathway. Diabetes Metab Syndr Obes 10:207-222 (2017). WB ; Rat . Read more (PubMed: 28652790) »
  • Lubieniecka JM  et al. Biomarkers for severity of spinal cord injury in the cerebrospinal fluid of rats. PLoS One 6:e19247 (2011). WB ; Rat . Read more (PubMed: 21559420) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab59524.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up