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Glutathione Peroxidase Assay Kit (ab102530)

Q&A (15)

Overview

  • Product nameGlutathione Peroxidase Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Platelets, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    0.5 mU/ml
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: all Mammals
  • Product overview

    In Abcam's Glutathione Peroxidase Assay Kit, GPx reduces Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH (easily measured at 340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.
    • Alternative names
        Glutathione PeroxidaseGlutathione Peroxidase Assay

    Applications

    Our Abpromise guarantee covers the use of ab102530 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Glutathione Peroxidase Assay Kit images

    • Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)
      Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)
      Example of standard curve obtained using ab102530
    • Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)
      Functional Studies - Glutathione Peroxidase Assay Kit (ab102530)
      Tests example obtained using ab102530

    Protocols

    References for Glutathione Peroxidase Assay Kit (ab102530)

    ab102530 has not yet been referenced specifically in any publications.

    Publishing research using ab102530 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Displaying 1 - 10 of 15 results for Abreviews and Q&A

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    Dear All,



    Please let us know if the product ab102530-Glutathione Peroxidase can be run on RAT samples.



    Early response requested.


    Sincere regards,

    Thank you for your inquiry.

    I am happy to confirm that ab102530 (Glutathione Peroxidase Assay Kit) is suitable for all mammalian samples, including rat samples.

    I wish your customer good luck with their experiments.

    Thank you for your response regarding my query. However, there is no point in issuing me a positive control as I have already done the experiment, using the other reagents in the kit. I do not have not enough lysate to repeat the assay. If I repeat the wh...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caus...

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    Inquiry: Hi, I recently tried your GPX assay and found that I got a response from my cells using a density of apprx 2.5 x 10e6. I haven't however got a response for the positive control, using 10ul. I have tried this twice. If you could tell me the concen...

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    I have recieved a reply from the lab who confirm that there was a problem with the positive control from this lot. Unfortunately the positive control from this lot lost activity for unforeseen reasons
    I am sorry this product did not perform as stated...

    Read More

    Inquiry: Hi, I recently tried your GPX assay and found that I got a response from my cells using a density of apprx 2.5 x 10e6. I haven't however got a response for the positive control, using 10ul. I have tried this twice. If you could tell me the concen...

    Read More

    Thank you for contacting us.
    I am currently in contact with the lab who are rechecking the positive control from this lot.
    In the meantime, they would like to know what difference you got in the absorbance from the vector alone and the GPx expre...

    Read More

    Hello,

    I would like to ask you about possibility to obtain GPx Positive Control separately. My second question is, what is the catalytic concentration of this control material. Can I have an application sheet?

    Thanks a lot

    Read More

    Thank you for your inquiry.

    I am sorry to confirm that we do not offer the positive control separately for purchase.

    I am sorry to not have a positive answer on this occasion and wish you a good weekend.

    Thank you for your reply.

    I wish to detect the presence of antioxidant enzymes in the tissue (glutathione peroxidase and reductase, SOD and catalase most important) but also the quantification or activity of each enzyme is important. Essentially...

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    Thank you for contacting us.

    Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use are w...

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    I am inquiring about your total antioxidant capacity assay kit or similar.

    I have frozen placental tissue from rabbit experiments and I am wishing to examine markers of oxidative stress.
    The most important markers for me are glutathione perox...

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    Thank you for contacting us.

    Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

    I will be looking forward to hearing from you soon.

    Hello,
    My order# from Abcamencountered problems and is currently experiencing major delays with customs in my country. I want to inquire, will the products in this order still work if they were put for more than 4 days in room temperature?
    Here ...

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    Thank you for contacting us.

    Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storin...

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    Thank you for your email and for the clarification. However, the fractionation kit ab65320 is for use in cells and not plasma, so would not be relevant for our plasma samples. So is this kit still able to measure both Cu/Zn and Mn SOD in our plasma sample...

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    Thank you for contacting us.

    Please do not get confused with cytosol and mitochondrial SODs for plasma; the information I had provided was for general cells samples. I had missed the fact you are going to use plasma and plasma does not contain ce...

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    - 720 samples
    - Interested in ab65354, ab83464, ab102530 and ab118970
    - WIll use heparin containing plasma samples
    - Activity kits

    Thank you for contacting us.

    I can confirm that the kit ab65354 will detect all SODs (Cu/Zn, Mn and FeSOD).

    If it is desired to measure SOD activity from cytosol and mitochondria separately,these can be separated by using ab65320 Mitocho...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"