Glutathione Peroxidase Assay Kit (ab102530)
- Product nameGlutathione Peroxidase Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Platelets, Other biological fluids, Tissue Extracts
- Assay typeEnzyme activity
- Sensitivity0.5 mU/ml
- Assay time0h 40m
- Species reactivityReacts with: Rat, Human
Predicted to work with: all Mammals
- Product overview
In Abcam's Glutathione Peroxidase Assay Kit, GPx reduces Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH (easily measured at 340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.
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The Glutathione Peroxidase (GPx, EC 18.104.22.168) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests 1 kit Cumene Hydroperoxide Yellow 1 vial 1 vial Glutathione Brown 1 vial 1 vial Glutathione Reductase Green 1 vial 1 vial GPx Assay Buffer WM 1 x 25ml 1 x 25ml GPx Positive Control Red 1 vial 1 vial NADPH Blue 1 vial 1 vial
- Kits/ Lysates/ Other
- Cell Metabolism Kits
- Coenzyme and cofactor assay kits
- Glutathione assay kits
- Glutathione PeroxidaseGlutathione Peroxidase Assay
Our Abpromise guarantee covers the use of ab102530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Glutathione Peroxidase Assay Kit images
Example of standard curve obtained using ab102530
Tests example obtained using ab102530
References for Glutathione Peroxidase Assay Kit (ab102530)
ab102530 has not yet been referenced specifically in any publications.