Glutathione Reductase (GR) Assay Kit (ab83461)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 mU/ml
Overview
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Product name
Glutathione Reductase (GR) Assay Kit -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Sensitivity
> 0.1 mU/ml -
Range
0.1 mU/ml - 40 mU/ml -
Assay time
0h 40m -
Product overview
Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
Since Glutathione Reductase has significantly higher concentrations in cells (mM range) compared to Thioredoxin Reductase (µM range), we predict that ab83461 will detect mostly GR activity in samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K761 Glutathione Reductase Activity Colorimetric Assay Kit. K761-200 is the same size as the 200 test size of ab83461.
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
Related products
Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer XLI 1 x 100ml Catalase 1 x 1ng DTNB 1 vial GR Positive Control 1 vial GSSG 1 vial NADPH Generating Mix 2 vials Substrate V 1 x 1ml TNB Standard 1 x 1ng -
Research areas
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Relevance
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. -
Cellular localization
Cytoplasmic and Mitochondrial -
Alternative names
- EC 1.8.1.7
- Glutathione reductase mitochondrial
- GR
see all
Images
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ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
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Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).
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Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)
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Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.
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Glutathione Reductase assay time line using ab83461.
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TNB Standard Curve using ab83461.
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab83461 has been referenced in 29 publications.
- Salama SA et al. Galangin attenuates cadmium-evoked nephrotoxicity: Targeting nucleotide-binding domain-like receptor pyrin domain containing 3 inflammasome, nuclear factor erythroid 2-related factor 2, and nuclear factor kappa B signaling. J Biochem Mol Toxicol 36:e23059 (2022). PubMed: 35384154
- Likittrakulwong W et al. Effects of Intramuscular Injections of Vitamins AD3E and C in Combination on Fertility, Immunity, and Proteomic and Transcriptomic Analyses of Dairy Cows during Early Gestation. BioTech (Basel) 11:N/A (2022). PubMed: 35822793
- Pesti-Asbóth G et al. Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato in vitro. Front Plant Sci 13:979141 (2022). PubMed: 36247572
- Mohamed ME & Younis NS Ameliorative Effect of D-Carvone against Hepatic Ischemia-Reperfusion-Induced Injury in Rats. Life (Basel) 12:N/A (2022). PubMed: 36294936
- Alkhalifah EAR et al. Cardamom Extract Alleviates the Oxidative Stress, Inflammation and Apoptosis Induced during Acetaminophen-Induced Hepatic Toxicity via Modulating Nrf2/HO-1/NQO-1 Pathway. Curr Issues Mol Biol 44:5390-5404 (2022). PubMed: 36354677