Glutathione Reductase (GR) Assay Kit (ab83461)
- Product nameGlutathione Reductase (GR) Assay Kit
- Tests200 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
- Assay typeEnzyme activity
- Sensitivity> 0.1 mU/ml
- Range0.1 mU/ml - 40 mU/ml
- Assay time0h 40m
- Product overview
Abcam's Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
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Glutathione Reductase (GR, EC 22.214.171.124) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 200 tests Catalase (lyophilized) Clear 1 x 1ng DTNB (lyophilized) Red 1 vial GR Assay Buffer NM 1 x 100ml GR Positive Control (10 mU; lyophilized) Green 1 vial GSSG (lyophilized) Yellow 1 vial Hydrogen peroxide Orange 1 x 1ml NADPH-GNERAT™ (lyophilized) Blue 2 vials TNB Standard (2.5 µmol) Brown 1 x 1ng
- EC 126.96.36.199Glutathione reductase mitochondrialGR
Our Abpromise guarantee covers the use of ab83461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Glutathione Reductase (GR) Assay Kit images
TNB Standard Curve using ab83461.
Glutathione Reductase assay time line using ab83461.
References for Glutathione Reductase (GR) Assay Kit (ab83461)
ab83461 has not yet been referenced specifically in any publications.