Glutathione Reductase (GR) Assay Kit (ab83461)

Overview

  • Product nameGlutathione Reductase (GR) Assay Kit
  • Tests
    200 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.1 mU/ml
  • Range
    0.1 mU/ml - 40 mU/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, ?max = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceGlutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
  • Cellular localizationCytoplasmic and Mitochondrial
  • Alternative names
    • EC 1.8.1.7
    • Glutathione reductase mitochondrial
    • GR
    • GSR
    • MGC78522
    see all

Applications

Our Abpromise guarantee covers the use of ab83461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glutathione Reductase (GR) Assay Kit images

  • Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

  • Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

  • Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

  • Glutathione Reductase assay time line using ab83461.
  • TNB Standard Curve using ab83461.

Protocols

References for Glutathione Reductase (GR) Assay Kit (ab83461)

ab83461 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The procedure is given in protocol booklet.

http://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

Please thaw frozen tissue on ...

Read More

Thank you for contacting us.

Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use ar...

Read More

Thank you for contacting us.

Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

I will be looking forward to hearing from you soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"