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Glutathione Reductase (GR) Assay Kit (ab83461)

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Overview

  • Product nameGlutathione Reductase (GR) Assay Kit
  • Tests
    200 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.1 mU/ml
  • Range
    0.1 mU/ml - 40 mU/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
  • Cellular localizationCytoplasmic and Mitochondrial
    • Alternative names
        EC 1.8.1.7Glutathione reductase mitochondrialGR
        GSRMGC78522
      see all

    Applications

    Our Abpromise guarantee covers the use of ab83461 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Glutathione Reductase (GR) Assay Kit images

    • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      TNB Standard Curve using ab83461.
    • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Functional Studies - Glutathione Reductase Assay Kit (ab83461)
      Glutathione Reductase assay time line using ab83461.

    Protocols

    References for Glutathione Reductase (GR) Assay Kit (ab83461)

    ab83461 has not yet been referenced specifically in any publications.

    Publishing research using ab83461 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab83461 Submit a Question for ab83461

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    Inquiry: Hello, I am considering ordering Glutathione Reductase (GR) Assay Kit (ab83461) to study this enzyme activity. I have frozen rabbit placenta. Can you advise me of the details / protocol of preparing this tissue for this analysis (ie tissue extrac...

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    Thank you for contacting us.

    The procedure is given in protocol booklet.

    http://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

    Please thaw frozen tissue on ice...

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    Thank you for your reply.

    I wish to detect the presence of antioxidant enzymes in the tissue (glutathione peroxidase and reductase, SOD and catalase most important) but also the quantification or activity of each enzyme is important. Essentially...

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    Thank you for contacting us.

    Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use are w...

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    I am inquiring about your total antioxidant capacity assay kit or similar.

    I have frozen placental tissue from rabbit experiments and I am wishing to examine markers of oxidative stress.
    The most important markers for me are glutathione perox...

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    Thank you for contacting us.

    Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

    I will be looking forward to hearing from you soon.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"