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Anti-Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)

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Overview

Product name

Anti-Glutathione S Transferase kappa 1 antibody [EP1938Y]
See all Glutathione S Transferase kappa 1 products (3) ...

Description

Rabbit monoclonal [EP1938Y] to Glutathione S Transferase kappa 1

Specificity

This antibody reacts with Glutathione S Transferase kappa 1.

Tested applications

WB, IP, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues near the C-terminus of human Glutathione S Transferase kappa 1.

Positive control

Fetal liver lysate and human liver tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1938Y

Isotype

IgG

Research areas

Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

  • Western blot - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)Western blot - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)Immunohistochemistry (Paraffin-embedded sections) - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is strongly recommended for enhanced signal.
IP: 1/40.
WB: 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Is unsuitable for Flow Cyt.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the GST superfamily. Kappa family.

Cellular localization

Peroxisome.

Target information above from: UniProt accessionQ9Y2Q3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EC 2.5.1.18 antibody
  • Glutathione S transferase subunit 13 antibody
  • Glutathione S-transferase kappa 1 antibody
  • Glutathione S-transferase subunit 13 antibody
  • GST 13 13 antibody
  • GST 13-13 antibody
  • GST class kappa antibody
  • GST class-kappa antibody
  • GSTK1 1 antibody
  • Gstk1 antibody
  • GSTK1-1 antibody
  • GSTK1_HUMAN antibody
  • hGSTK1 antibody
see all

Database links

Anti-Glutathione S Transferase kappa 1 antibody [EP1938Y] images:

  Western blot - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)

Western blot - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)

Anti-Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759) at 1/5000 dilution + Fetal liver lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 25 kDa
Observed band size : 25 kDa

  Immunohistochemistry (Paraffin-embedded sections) - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)

Immunohistochemistry (Paraffin-embedded sections) - Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)

Immunohistochemical staining of paraffin-embedded human liver using ab52759 at a 1:100 dilution.

References for Anti-Glutathione S Transferase kappa 1 antibody [EP1938Y] (ab52759)

ab52759 has not yet been referenced specifically in any publications.

Publishing research using ab52759? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"