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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-Glutathione S Transferase kappa 1 antibody
See all Glutathione S Transferase kappa 1 products (3) ...
Description
Mouse polyclonal to Glutathione S Transferase kappa 1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length Human Glutathione S Transferase kappa 1 protein (BC001231).
Positive control
Human liver tissue lysate, Glutathione S Transferase kappa 1 transfected 293T cell line lysate and HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab89116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 25 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the GST superfamily. Kappa family.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionQ9Y2Q3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 2.5.1.18 antibody
- Glutathione S transferase subunit 13 antibody
- Glutathione S-transferase kappa 1 antibody
see all
Database links
- Entrez Gene: 373156 Human
- Omim: 602321 Human
- SwissProt: Q9Y2Q3 Human
- Unigene: 390667 Human
Anti-Glutathione S Transferase kappa 1 antibody images:
Western blot - Glutathione S Transferase kappa 1 antibody (ab89116)
Anti-Glutathione S Transferase kappa 1 antibody (ab89116) at 1 µg/ml + Human liver tissue lysate at 50 µg
Predicted band size : 25 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Western blot - Glutathione S Transferase kappa 1 antibody (ab89116)
All lanes : Anti-Glutathione S Transferase kappa 1 antibody (ab89116) at 1 µg/ml
Lane 1 : Glutathione S Transferase kappa 1 transfected 293T cell line lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 25 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Glutathione S Transferase kappa 1 antibody (ab89116)
ab89116, at 10 µg/ml staining Glutathione S Transferase kappa 1 in HeLa cells by Immunofluorescence.
References for Anti-Glutathione S Transferase kappa 1 antibody (ab89116)
ab89116 has not yet been referenced specifically in any publications.
Publishing research using ab89116? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"