Overview

  • Product nameAnti-Glutathione antibody [D8]
    See all Glutathione primary antibodies
  • Description
    Mouse monoclonal [D8] to Glutathione
  • SpecificityThis antibody reacts with glutathione-protein complexes under non-reducing conditions.
  • Tested applicationsIHC-FoFr, WB, IHC-Fr, IHC-P, Flow Cyt, IP, ELISA, ICC, ICC/IFmore details
  • Immunogen

    Glutathione conjugated to KLH

Properties

Applications

Our Abpromise guarantee covers the use of ab19534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 19833109
WB 1/1000. Use under non reducing condition. We recommend blocking with 5% milk (not BSA). While glutathione itself is too small to detect in WB, this antibody will detect all glutathionylated proteins. You may observe multiple bands at variable molecular weights depending on what proteins in your samples are glutathionylated.
IHC-Fr 1/100 - 1/200.
IHC-P Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceGlutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione is involved in detoxification, it binds to toxins, such as heavy metals, solvents, and pesticides, and transforms them into a form that can be excreted in urine or bile. It is also an important antioxidant, helping to maintain the -SH groups of proteins in their reduced form. Chronic functional glutathione deficiency is associated with glucose 6-phosphate dehydrogenase deficiency, immune disorders, an increased incidence of malignancies, and in the case of HIV disease, probably accelerated pathogenesis of the disease. Acute manifestations of functional glutathione deficiency can be seen in those who have taken an overdosage of acetaminophen (paracetamol). This results in depletion of glutathione in the hepatocytes, leading to liver failure and death.
  • Alternative names
    • Glutathione antibody
    • GSH antibody
    • Oxidised glutathione antibody
    • Reduced glutathione antibody

Anti-Glutathione antibody [D8] images

  • ab19534 staining Glutathione in human neutrophils by Immunocytochemistry/ Immunofluorescence.
    Samples were fixed with 4% (w/v) paraformaldehyde in PBS, permeabilized with PBS containing 0.5% (w/v) saponin and 0.1% (w/v) bovine serum albumin, and then blocked with 1% (w/v) bovine serum albumin in PBS for 1 hour. ab19534 was used at 5µg/ml for 2 hours at room temperature. After washing with PBS, cells were incubated with Alexa 488-conjugated anti-mouse IgG at a 1/200 dilution.
  • ab19534 at a 1/250 dilution detecting Glutathione in human monocytes by Flow Cytometry. An Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) secondary was used at a 1/500 dilution.

    See Abreview

  • ab19534 staining Glutathione in cultured murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab19534 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.

    See Abreview

  • ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.

References for Anti-Glutathione antibody [D8] (ab19534)

This product has been referenced in:
  • Inui T  et al. Association of p62/SQSTM1 Excess and Oral Carcinogenesis. PLoS One 8:e74398 (2013). IHC-P ; Human . Read more (PubMed: 24086340) »
  • Sun R  et al. Oxidative stress induced S-glutathionylation and proteolytic degradation of mitochondrial thymidine kinase 2. J Biol Chem 287:24304-12 (2012). WB . Read more (PubMed: 22661713) »

See all 7 Publications for this product

Product Wall




"It is technically challenging and we do not recommend it for quantitative analysis.

The best method developed so far is that of Sun and Kalloniatis, J Comp Neurol 494:686 -703, 2006.

We advise to consider this...

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I have been informed by the lab that the conjugation of KLH to glutathione was through the thiol group.

As requested I issued a credit note. The credit note ID xxxxxx.

As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID nu...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion.
I appreciate the time your customer has spent on these experiments and would be ...

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We have 2 antibodies that react with conjugated glutathione and 2 that are designed to react with free glutathione. I have looked at these products and it doesn't appear any of them have been specifically tested for reactivity to glutathione ...

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I'm sorry but at this time we don't have an antibody that reacts specifically to glutathione as a thioether.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 10 µg
Specification Liver
Treatment 10mM Diamide
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 35 µg
Specification HeLa cells
Treatment Diamide for 24h
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2012

Vielen Dank für Ihre Anfrage.

Glutathion ist nicht Spezies spezifisch, da es immer aus Cystein, Glutaminsaeure und Glycin besteht.

Wir haben ab19534 nicht speziell mit Rattenproben getestet, gehen aber davon aus, dass der Antik...

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Thank you for contacting us.

Glutathione itself will be too small to view in a WB, but the customer will be able to detect any proteins that are glutathionylated in his or her samples. The results will show multiple bands at varying molecular...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"