Anti-Glutathione antibody [D8] (ab19534)

Overview

• Product nameAnti-Glutathione antibody [D8]See all Glutathione primary antibodies ...
• Description
  Mouse monoclonal [D8] to Glutathione
• SpecificityThis antibody reacts with glutathione-protein complexes under non-reducing conditions.
• Tested applicationsIHC-FoFr, WB, IHC-Fr, IHC-P, Flow Cyt, IP, ELISA, ICC, ICC/IF more details
• Immunogen

  Glutathione conjugated to KLH

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberD8
• IsotypeIgG2a
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Antioxidants

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• RelevanceGlutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione is involved in detoxification, it binds to toxins, such as heavy metals, solvents, and pesticides, and transforms them into a form that can be excreted in urine or bile. It is also an important antioxidant, helping to maintain the -SH groups of proteins in their reduced form. Chronic functional glutathione deficiency is associated with glucose 6-phosphate dehydrogenase deficiency, immune disorders, an increased incidence of malignancies, and in the case of HIV disease, probably accelerated pathogenesis of the disease. Acute manifestations of functional glutathione deficiency can be seen in those who have taken an overdosage of acetaminophen (paracetamol). This results in depletion of glutathione in the hepatocytes, leading to liver failure and death.

• Alternative names
  Glutathione antibodyGSH antibodyOxidised glutathione antibodyReduced glutathione antibody

Anti-Glutathione antibody [D8] images

• 

  Immunocytochemistry/ Immunofluorescence - Glutathione antibody [D8] (ab19534)Image from Lim SY et al, J Biol Chem. 2010 May 7;285(19):14377-88. Epub 2010 Mar 11, Fig 4.
  ab19534 staining Glutathione in human neutrophils by Immunocytochemistry/ Immunofluorescence.
  
  Samples were fixed with 4% (w/v) paraformaldehyde in PBS, permeabilized with PBS containing 0.5% (w/v) saponin and 0.1% (w/v) bovine serum albumin, and then blocked with 1% (w/v) bovine serum albumin in PBS for 1 hour. ab19534 was used at 5µg/ml for 2 hours at room temperature. After washing with PBS, cells were incubated with Alexa 488-conjugated anti-mouse IgG at a 1/200 dilution.
• 

  Flow Cytometry - Anti-Glutathione antibody [D8] (ab19534)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
  ab19534 at a 1/250 dilution detecting Glutathione in human monocytes by Flow Cytometry. An Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) secondary was used at a 1/500 dilution.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
  ab19534 staining Glutathione in cultured murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab19534 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (ab19534)
  ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
  The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.