Overview

  • Product name
    Glycine Assay Kit (Fluorometric)
  • Detection method
    Fluorescent
  • Sample type
    Saliva, Urine, Serum, Plasma, Cell Lysate, Tissue Lysate
  • Assay type
    Quantitative
  • Sensitivity
    1 µM
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    Glycine Assay Kit (Fluorometric) (ab211100) provides a provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentrations of glycine (GLY) in multiple biological samples such as cell and tissue lysates and biological fluids. In this assay, Glycine is oxidized in the presence of GLY Enzyme Mix is converted to an intermediate, which reacts with the fluorescent probe to generate a strong stable signal at Ex/Em = 535/587 nm. The intensity of the signal is directly proportional to the amount of GLY in the sample.


    The reaction is specific and other amino acids do not interfere with the assay. The assay can detect as little as 1 µM of Glycine in a variety of biological samples.

  • Notes

    Glycine (GLY, G) is one of the 20 standard amino acids commonly found in proteins. Glycine’s side chain is a hydrogen substituent, which makes it the smallest proteogenic and the only non-chiral amino acid. Basic functions of Glycine include the participation in the synthesis of creatine, glutathione, heme groups, and conjugated bile acids (bile salts). It is also present as one of the most abundant residues in the triple-helical structure of collagen.

    Glycine acts as a glucogenic amino acid by regulating sugar levels in blood. Therefore, glycine supplementation has been used in patients suffering anemia, hypoglycemia and chronic fatigue. Glycine possesses both inhibitory and excitatory neurotransmitter functions in the brain stem and spinal cord. In cancer cells, glycine consumption is highly correlated to cancer cell proliferation via purine synthesis. Glycine uptake in cancer cell studies supports the role of this amino acid in tumorigenesis and malignancy.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    GLY Assay Buffer 1 x 25ml
    GLY Developer (200 µg) 1 vial
    GLY Enzyme Mix (600 mU) 1 vial
    GLY Probe 1 x 400µl
    GLY Standard (10 µM) 1 vial

Applications

Our Abpromise guarantee covers the use of ab211100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Typical Glycine standard calibration curve.

  • Estimation of Glycine in human serum, saliva, and urine. Samples were deproteinized using 10 kD spin column (ab93349) and diluted with GLY Assay Buffer prior to performing assay: (serum 1:64 dilution; saliva 1:32 dilution; urine 1:128 dilution). 25 µL of each diluted sample was spiked with 0.3 nmol of Glycine Standard and assayed following the kit protocol. Glycine concentrations in serum (224 ± 21µM), saliva (149 ± 7 µM) and urine (54 ± 4 µM/mM creatinine).

Protocols

References

ab211100 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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