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Product Name
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Glycine antibody
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See all Glycine antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Glycine
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Immunogen
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Synthetic glycine conjugated to protein carrier.
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Species Reactivity
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not applicable
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Specificity
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This antibody targets conjugated glycine. Using a conjugate glycine-Protein Carrier (Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Glycine-G-(Pc) = 1 ß-Alanine-G-(Pc) = 1/100 GABA-G-(Pc) = 1/500 Taurine-G-(Pc) = 1/1,000 Aspartate-G-(Pc) = 1/20,000 Glutamate-G-(Pc) = 1/>20,000 Glycine = 1/>50,000
G = Glutaraldehyde § Glycine-G-(Pc) concentration/unconjugated or conjugated amino acids concentration at half displacement.
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Tested applications
(see key)
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ELISA, ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/1000 - 1/5000. ICC: 1/1000 - 1/5000. (Not recommended for use with formaldehyde). WB: 1/1000 - 1/2000.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Signal Transduction >> Metabolism >> Amino Acids Neuroscience >> Neurotransmitter >> Amino Acids >> Glycine
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Relevance
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Defects in GLDC are a cause of nonketotic hyperglycinemia (NKH), also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. The degredation of glycine is catalised by the glycine cleavage system. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; carbondioxide is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. The glycine cleavage system is composed of four proteins: P, T, L and H.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, Whole serum. pH 7.4 Material safety datasheet (MSDS) for this product: Thimerosal MSDS
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Purification notes
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Antiserum previously preabsorbed on protein carriers and purified. Antisera antibody was lyophilized and then reconstituted in buffer.
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Glycine antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Glycine
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Glycine antibody - more information
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