Glycine antibody (ab37087)

Product Name 

Glycine antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Glycine

Immunogen 

Synthetic glycine conjugated to protein carrier.

Species Reactivity 

not applicable

Specificity 

This antibody targets conjugated glycine.
Using a conjugate glycine-Protein Carrier (Pc), antibody specificity was performed with an ELISA test by
competition experiments with the following compounds:

Glycine-G-(Pc) = 1
ß-Alanine-G-(Pc) = 1/100
GABA-G-(Pc) = 1/500
Taurine-G-(Pc) = 1/1,000
Aspartate-G-(Pc) = 1/20,000
Glutamate-G-(Pc) = 1/>20,000
Glycine = 1/>50,000

G = Glutaraldehyde
§ Glycine-G-(Pc) concentration/unconjugated or conjugated amino acids concentration at half
displacement.

Tested applications 
(see key)

ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000 - 1/5000.
ICC: 1/1000 - 1/5000. (Not recommended for use with formaldehyde).
WB: 1/1000 - 1/2000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmitter >> Amino Acids >> Glycine

Relevance 

Defects in GLDC are a cause of nonketotic hyperglycinemia (NKH), also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms.
The degredation of glycine is catalised by the glycine cleavage system. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; carbondioxide is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.
The glycine cleavage system is composed of four proteins: P, T, L and H.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, Whole serum. pH 7.4

Material safety datasheet (MSDS) for this product:
Thimerosal MSDS

Purification notes 

Antiserum previously preabsorbed on protein carriers and purified. Antisera antibody was lyophilized and then reconstituted in buffer.

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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