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Anti-Glycine antibody (ab37087)

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Overview

Product name

Anti-Glycine antibody
See all Glycine products (2) ...

Description

Rabbit polyclonal to Glycine

Specificity

This antibody targets conjugated glycine. Using a conjugate glycine-Protein Carrier (Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Glycine-G-(Pc) = 1 ß-Alanine-G-(Pc) = 1/100 GABA-G-(Pc) = 1/500 Taurine-G-(Pc) = 1/1,000 Aspartate-G-(Pc) = 1/20,000 Glutamate-G-(Pc) = 1/>20,000 Glycine = 1/>50,000 G = Glutaraldehyde § Glycine-G-(Pc) concentration/unconjugated or conjugated amino acids concentration at half displacement.

Tested applications

ELISA, ICC, WBmore details

Cross reactivity

not applicable

Immunogen

Synthetic glycine conjugated to protein carrier.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, ddH20

Purity

Whole antiserum

Purification notes

Antiserum previously preabsorbed on protein carriers and purified. Antisera antibody was lyophilized and then reconstituted in buffer.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmitter >> Amino Acids >> Glycine

Applications

Show applications key

Our Abpromise guarantee covers the use of ab37087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

  • ICC

     

  • WB

     

Application notes

ELISA: 1/1000 - 1/5000.
ICC: 1/1000 - 1/5000. (Not recommended for use with formaldehyde).
WB: 1/1000 - 1/2000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Defects in GLDC are a cause of nonketotic hyperglycinemia (NKH), also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. The degredation of glycine is catalised by the glycine cleavage system. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; carbondioxide is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. The glycine cleavage system is composed of four proteins: P, T, L and H.

Alternative names

  • glycine antibody

References for Anti-Glycine antibody (ab37087)

ab37087 has not yet been referenced specifically in any publications.

Publishing research using ab37087? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"