Overview

  • Product name
    Glycogen Assay Kit II (Colorimetric)
    See all Glycogen kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    > 4 µg/ml
  • Range
    4 µg/ml - 40 µg/ml
  • Species reactivity

    Predicted to work with: a wide range of other species, Mammal
  • Product overview

    Glycogen Assay Kit II (Colorimetric) (ab169558) provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless probe to a colored product with strong absorbance at 450 nm. This high-throughput suitable assay kit can detect 4 - 40 µg/ml of Glycogen in samples.

  • Notes

    Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of a-1,4 linkage with a-1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Development Enzyme Mix (lyophilized) Green 1 vial
    Glycogen Development Buffer 1 x 25ml
    Glycogen Hydrolysis Buffer 1 x 25ml
    Glycogen Standard (2.0 mg/ml) Yellow 1 x 100µl
    Hydrolysis Enzyme Mix (lyophilized) Blue 1 vial
    Probe 1 vial
  • Research areas
  • Relevance
    Glycogen is the primary short term energy storage molecule in animals. It is synthesized primarily in the liver and muscle. Glycogen is a highly branched polymer of glucose molecules, connected with an alpha-1,4 linkage, branching via an alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.

Applications

Our Abpromise guarantee covers the use of ab169558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Glycogen measured in cell lysates showing quantity (�g) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.

  • Glycogen measured in tissue lysates showing quantity (�g) per mg of extracted protein.

    Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

Protocols

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

I can confirm that the kit is not species specific so it should be suitable for mouse. It has also been tested for tissue samples, so this wil also be covered by the guarantee.


http://www.abcam.com/con...

Read More

ab65620, Glycogen Assay Kit, can detect glycogen 0.0004 to 2 mg/ml. This kit can be used in both colorimetric and fluorometric applications. In this assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up