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Overview

  • Product nameGlycogen Assay KitSee all Glycogen kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue
  • Assay typeQuantitative
  • Sensitivity
    > 0.4 µg/ml
  • Range
    0.4 µg/ml - 2000 µg/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glycogen Assay Kit is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in alpha-1,4 linkage, with branching via alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlycogen is the primary short term energy storage molecule in animals. It is synthesized primarily in the liver and muscle. Glycogen is a highly branched polymer of glucose molecules, connected with an alpha-1,4 linkage, branching via an alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.

    • Applications

    Our Abpromise guarantee covers the use of ab65620 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Glycogen Assay Kit images

    • Functional Studies - Glycogen Assay Kit (ab65620)
      Functional Studies - Glycogen Assay Kit (ab65620)
      Glycogen Standard Curve: Assays were performed following the kit protocol.

    Protocols

    References for Glycogen Assay Kit (ab65620)

    ab65620 has not yet been referenced specifically in any publications.

    Publishing research using ab65620 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab65620

    Displaying 1 - 10 of 39 results for Q&A

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    Inquiry: Hello, I used several times your kits for the determination of glycogen in tissues homogenized in water and got good results. Now I need to use Levamisol 10mM for homogenization and I wonder if there may be interference with this kit. thank ...

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    Thank you for contacting us.

    This kit was optimized using water, and unfortunately we don't have any data on whether Levamisol might work. This would have to be tested.

    I hope this information is helpful to you. Please do not hesitat...

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    Inquiry: Hi, I used your Glycogen Assay kit for several months. I need to change buffer for the homogenization because I need the same homogenate for other dosing. I would like to use PBS or metaphosphoric acid (0.5-2%) instead of H2O. Do you know if...

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    Thank you for contacting us.

    PBS can be used in place of water for homgenizing.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    I want to assay glycogen content in the cell using abcam kit. I would like to know whether I can cell lysis buffer to lyse cells and whether lysis buffer  will affect the measurement result of glycogen. If i can use lysis buffer, can you let me know which...

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    Are you inquiring about ab65620? If so, you will not need to use any lysis buffers with this product. For tissue or cell samples with this kit you will homogenize 10^6 cells or 10 mg tissue with 200 μl dH₂O on ice. Then boil the homogenates for 5 min t...

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    Please advise the shipping conditions of ab65620. Is it 4C or -20C?
    Thanks in advance.
    Best Regards,

    Thank you for contacting us.
    The shipping temperature for this kit is +4C and for storage temperature please refer to the protocol.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or inf...

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    Dear xxxxx,

    Thank you for your prompt reply.

    This is a general request from our customer that purchases ab65620. This time Hydrolysis Enzyme Mix and Development Enzyme Mix ran out before the rest of the components.

    Meanwhile, th...

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    I am sorry for the delay in getting back to you.
    The components the customer has requested have now been added to the catalogue under the catalogue numbers ab154150 and ab154151. They should be available to view on the website by tomorrow.
    The k...

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    Dear Tech Support Team,

    Please advise if it is possible to purchase single components of ab65620 Abcam Glycogen Assay Kit.

    Thanks in advance.

    Best Regards,

    Thank you for contacting us and your interest in our products.
    It may be possible to provide the components of the Glycogen Assay Kit (ab65620), however I will need to investigate this further. Is it a particular component the customer is interested ...

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    Phone call requesting further information in regards to how the glucose oxidation and subsequent reaction with the Oxired probe of the Glycogen Assay Kit (ab65620) may be affected by hypoxic conditions.

    Many thanks for contacting us and sorry for the delay in getting back to you. I now have further information to share with you in regards to the Glycogen Assay Kit (ab65620).

    The kit uses for its detection an OxiRed probe. Therefore any H2O2 pre-...

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    Can you sell the components of the glycogen assay kit individually? How long do the enzyme solutions last for? Thanks

    Thank you for contacting us. We do not currently sell the components for our glycogen assay kit separately. The enzyme solutions will be stable for at least two months when stored at -20C. I hope this helps, please let me know if you need any additiona...

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    die Kits sind gut und schnell bei uns gelandet. Vielen Dank !
    Jetzt habe ich eine Frage zur Durchführung, im Assay Protocol steht beschrieben, dass die Proben bei 13000 rpm zentrifugiert werden sollen. Auf welche Zentrifuge bezieht sich diese An...

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    Vielen Dank für Ihre Anfrage.

    Die Zentrifugationsschritte in diesem Protokoll sollen alle in einer Tischzentrifuge (Röhrchengröße 1.5ml) stattfinden.

    13000rpm entspricht bei Standard Tischzentrifugen (Microcentrifu...

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    Dear Supplier,

    Our customer would like to find out if following kits are suitable for his use. He would like to measure ATP, chelatable iron and glycogen in rat liver tissues and chelatable iron in rat liver perfusate samples.:

    ab833...

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    Thank you for your enquiry.

    I can confirm you can use perfused liver samples with all these kits.

    ab83366 Iron Assay Kit will detect chelatable iron.

    The kits are suitable for all mammalian species.

    I hope this ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"