Overview

  • Product nameGlycogen Assay KitSee all Glycogen kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue
  • Assay typeQuantitative
  • Sensitivity
    > 0.4 µg/ml
  • Range
    0.4 µg/ml - 2000 µg/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Glycogen Assay Kit is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in alpha-1,4 linkage, with branching via alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceGlycogen is the primary short term energy storage molecule in animals. It is synthesized primarily in the liver and muscle. Glycogen is a highly branched polymer of glucose molecules, connected with an alpha-1,4 linkage, branching via an alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.

Applications

Our Abpromise guarantee covers the use of ab65620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glycogen Assay Kit images

  • Glycogen Standard Curve: Assays were performed following the kit protocol.

Protocols

References for Glycogen Assay Kit (ab65620)

This product has been referenced in:
  • Choi S  et al. Suppression of autophagic activation in the mouse uterus by estrogen and progesterone. J Endocrinol 221:39-50 (2014). Read more (PubMed: 24443716) »
  • Reina A  et al. Shifts in the distribution of mass densities is a signature of caloric restriction in Caenorhabditis elegans. PLoS One 8:e69651 (2013). Read more (PubMed: 23922767) »

See all 3 Publications for this product

Product Wall

Cells within collagen or Matrigel matrix are not ideal since when you homogenize the samples in the matrix and boil them, the matrix or components of the matrix can come along with the sample. This can affect the assay. Without knowing exactly what goe...

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If you are using something like Matrigel for culturing the primary hepatocytes, then it is essential to remove the gel and collect the cells before proceeding with the homogenization and boiling to extract the glycogen. Liver cells store glycogen and h...

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The fluorescent signal is quite stable for several hours when protected from light. However, we recommend measuring the plate immediately after incubation for the best results.

ab65620, Glycogen Assay Kit, can detect glycogen 0.0004 to 2 mg/ml. This kit can be used in both colorimetric and fluorometric applications. In this assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a...

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For 100-150mg of a tough tissue like muscle, I would definitely recommend using the KOH method. You can also consult the literature for any other glycogen extraction method that may be preferable.



I can confirm that glucose generates background readings. Since glucose is present in your sample, we strongly recommend to do a glucose control without the addition of hydrolysis enzyme to determine the level of glucose background in your sa...

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This kit was optimized using water, and unfortunately we don't have any data on whether Levamisol might work. This would have to be tested.

I hope this information is helpful to you. Please do not hesi...

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PBS can be used in place of water for homgenizing.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Are you inquiring about ab65620? If so, you will not need to use any lysis buffers with this product. For tissue or cell samples with this kit you will homogenize 10^6 cells or 10 mg tissue with 200 μl dH₂O on ice. Then boil the homogenates for 5 mi...

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The shipping temperature for this kit is +4C and for storage temperature please refer to the protocol.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"