Glycogen Assay Kit (ab65620)
- Product nameGlycogen Assay KitSee all Glycogen kits ...
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue
- Assay typeQuantitative
- Sensitivity> 0.4 µg/ml
- Range0.4 µg/ml - 2000 µg/ml
- Assay time0h 40m
- Product overview
Abcam's Glycogen Assay Kit is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.
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Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in alpha-1,4 linkage, with branching via alpha-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
- Storage bufferPreservative: None
Constituents: 0.1% Triton-X-100, DMSO
Components Identifier 100 tests Development Buffer WM 1 x 25ml Development Enzyme Mix (lyophilized) Green 1 vial Glycogen Standard (2.0 mg/ml) Yellow 1 x 100µl Hydrolysis Buffer NM 1 x 25ml Hydrolysis Enzyme Mix (lyophilized) Blue 1 vial OxiRed Probe Red 1 x 200µl
- Research Areas
Our Abpromise guarantee covers the use of ab65620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Glycogen Assay Kit images
Glycogen Standard Curve: Assays were performed following the kit protocol.
References for Glycogen Assay Kit (ab65620)
ab65620 has not yet been referenced specifically in any publications.