Anti-Glycogen synthase 1 (phospho S645) antibody (ab53691)

Overview

• Product nameAnti-Glycogen synthase 1 (phospho S645) antibodySee all Glycogen synthase 1 primary antibodies ...
• Description
  Rabbit polyclonal to Glycogen synthase 1 (phospho S645)
• Specificityab53691 detects endogenous levels of Glycogen Synthase only when phosphorylated at serine 645.
• Tested applicationsELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthetic phosphopeptide derived from human Glycogen Synthase around the phosphorylation site of serine 645 (P-P-S^P-P-S).

• Positive controlHuman skeletal muscle tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab53691 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

Applications

Target

• FunctionTransfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
• PathwayGlycan biosynthesis; glycogen biosynthesis.
• Involvement in diseaseDefects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
• Sequence similaritiesBelongs to the glycosyltransferase 3 family.

Target information above from: UniProt accession P13807 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2997 Human
  • Entrez Gene: 2997 Human
  • Entrez Gene: 2997 Human
  • Entrez Gene: 14936 Mouse
  • Entrez Gene: 690987 Rat
  • Omim: 138570 Human
  • SwissProt: P13807 Human
  • SwissProt: P13807 Human

  • SwissProt: P13807 Human
  • SwissProt: Q8VEB0 Mouse
  • SwissProt: Q9Z1E4 Mouse
  • SwissProt: A2RRU1 Rat
  • Unigene: 386225 Human
  • Unigene: 275654 Mouse
  • Unigene: 95278 Rat

  see all

• Alternative names
  EC 2.4.1.11 antibodyGlycogen antibodyGlycogen [starch] synthase antibody

  Glycogen synthase 1 (muscle) antibodyGlycogen synthase 1 antibodyGlycogen synthase1 antibodyGSY antibodyGYS 1 antibodyGYS antibodyGYS1 antibodyGYS1_HUMAN antibodymuscle antibodyStarchsynthase muscle antibody

  see all

Anti-Glycogen synthase 1 (phospho S645) antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - Glycogen synthase 1 (phospho S645) antibody (ab53691)
  ab53691 at 1/50 dilution staining Glycogen Synthase 1 in human skeletal muscle by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Glycogen synthase 1 (phospho S645) antibody(ab53691)
  IHC image of ab53691 staining in human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53691, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.