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Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers. This product combines Glycolysis Assay [Extracellular acidification] (ab197244) and Glycolysis Stress Test Companion Assay (ab222945) in one practical kit for single purchase.
The assay uses 2-Deoxyglucose (2-DG) and oligomycin to facilitate an in-depth analysis of cellular glycolytic flux: 2-DG blocks glycolysis through competitive hexokinase inhibition allowing quantification of non-glycolytic extracellular acidification (ECA); oligomycin inhibits ATP synthase, therefore preventing aerobic ATP generation and forcing cells to increase glycolytic flux to meet ATP demand. The levels of such compensatory glycolysis achievable under test conditions can reveal underlying glycolytic perturbation not evident under basal conditions and facilitates assessment of ATP-demand limited glycolysis capability. Glucose oxidase is included in the kit to provide a convenient cell-free ECA assay signal control.
Glycolysis is a critical ATP generating pathway in eukaryotic cells and plays a central role in numerous pathologies including cancer, diabetes and neurodegenerative disorders. The ability to conveniently characterize the glycolytic profile of in vitro cell models used in these disease areas and to determine how specific interventions such as nutrition deprivation, hypoxia or drug treatment impact the profile is therefore of considerable utility.
|2-DG||1 x 600µl|
|Glucose||1 x 720mg|
|Glucose Oxidase Signal Control||1 x 112.5µg|
|Glucose-free Respiration Buffer||1 tablet|
|Glycolysis Assay Reagent (lyophilized)||1 vial|
|Oligomycin||1 x 10.4µg|
|Respiration Buffer||1 tablet|
Our Abpromise guarantee covers the use of ab222946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab222946 has not yet been referenced specifically in any publications.