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A synthetic peptide corresponding to residues in Human Glycophorin A
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab129024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).|
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250.
|Flow Cyt||1/90. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
ab129024 staining Glycophorin A in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/2500). ab97051(1/500) HRP-conjugated goat anti-rabbit IgG(H&L) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Overlay histogram showing K562 cells stained with unpurified ab129024 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129024, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab129024, unpurified, at 1/100 dilution staining Glycophorin A in formalin fixed paraffin embedded Human spleen tissue by immunohistochemistry.
ab129024, unpurified, showing positive staining in Thyroid gland erythrocytes tissue.
ab129024, unpurified, showing negative staining in Normal brain tissue.
ab129024, unpurified, showing positive staining in Normal colon erythrocytes tissue.
ab129024, unpurified, showing positive staining in Normal kidney erythrocytes tissue.
ab129024, unpurified, showing positive staining in Normal placenta erythrocytes tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"