Anti-Glycophorin A antibody [EPR8200] (ab129024)

Overview

• Product nameAnti-Glycophorin A antibody [EPR8200]See all Glycophorin A primary antibodies ...
• Description
  Rabbit monoclonal [EPR8200] to Glycophorin A
• Tested applicationsWB, IP, IHC-P, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues in Human Glycophorin A

• Positive controlFetal liver lysate, Human lung tissue, Human spleen tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Dissociation constant (Kd) Kd = 2.38 x 10^-10 M
• Storage bufferpH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR8200
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Blood
  • Blood Cell Antigens
  • RBC Antigens
  • Blood Group Antigens

Applications

Target

• FunctionGlycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
• Sequence similaritiesBelongs to the glycophorin A family.
• Post-translational
  modificationsThe major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
• Cellular localizationCell membrane. Appears to be colocalized with SLC4A1.

Target information above from: UniProt accession P02724 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2993 Human
  • Entrez Gene: 14934 Mouse
  • Omim: 111300 Human
  • SwissProt: P02724 Human
  • SwissProt: P14220 Mouse
  • Unigene: 434973 Human
  • Unigene: 13123 Mouse

• Alternative names
  AI853584 antibodyBlood group--MN locus antibodyCD_antigen=CD235a antibody

  CD235a antibodyGLPA_HUMAN antibodyGlycophorin A (MNS blood group) antibodyGlycophorin A antibodyGlycophorin A, included antibodyGlycophorin-A antibodyGlycophorinA antibodyGPA antibodyGPErik antibodyGpMiIII antibodyGPSAT antibodyGYPA antibodyGYPA, included antibodyHGpMiIII antibodyHGpMiV antibodyHGpMiX antibodyHGpMiXI antibodyHGpSta(C) antibodyMN antibodyMN sialoglycoprotein antibodyMNS antibodyPAS-2 antibodyPAS2 antibodySialoglycoprotein alpha antibody

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Anti-Glycophorin A antibody [EPR8200] images

• 

  Western blot - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  Anti-Glycophorin A antibody [EPR8200] (ab129024) at 1/1000 dilution + Fetal liver lysate at 10 µg
  
  Predicted band size : 16 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  ab129024, at 1/100 dilution staining Glycophorin A in formalin fixed paraffin embedded Human lung tissue by immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (ab129024)
  ab129024, at 1/100 dilution staining Glycophorin A in formalin fixed paraffin embedded Human spleen tissue by immunohistochemistry.
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glycophorin A antibody [EPR8200](ab129024)
  ab129024 showing positive staining in Thyroid gland erythrocytes tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glycophorin A antibody [EPR8200](ab129024)
  ab129024 showing negative staining in Normal brain tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glycophorin A antibody [EPR8200](ab129024)
  ab129024 showing positive staining in Normal colon erythrocytes tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glycophorin A antibody [EPR8200](ab129024)
  ab129024 showing positive staining in Normal kidney erythrocytes tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Glycophorin A antibody [EPR8200](ab129024)
  ab129024 showing positive staining in Normal placenta erythrocytes tissue.
  
  
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  Other-Anti-Glycophorin A antibody [EPR8200](ab129024)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D