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Anti-Glycoprotein hormones (alpha 5) antibody [INN-hFSH-158] (ab11386)

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Overview

  • Product nameAnti-Glycoprotein hormones (alpha 5) antibody [INN-hFSH-158]See all Glycoprotein hormones (alpha 5) primary antibodies ...
  • Description
    Mouse monoclonal [INN-hFSH-158] to Glycoprotein hormones (alpha 5)
  • SpecificityAb11386 reacts with the alpha 5 epitope on the alpha subunit of hCG, hLH, hFSH, hISH and the free alpha subunit.It does not react with hCGB, hCGBcf, hCGBn, ctp hCGB or hLHB.
  • Tested applicationsELISA, RIA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human FSH.

  • General notesThe alpha 5 epitope is destroyed upon iodination.

Properties

Applications

Our Abpromise guarantee covers the use of ab11386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
RIA RIA: Use at an assay dependent dilution.

Target

  • RelevanceThe four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The alpha subunit belongs to the glycoprotein hormones alpha chain family.
  • Cellular localizationSecreted
  • Database links
    • Alternative names
        Anterior pituitary glycoprotein hormones common subunit alpha antibodyCG alpha antibodyCGA antibody
        Choriogonadotropin alpha chain antibodyChorionic gonadotrophin subunit alpha antibodyChorionic gonadotropin, alpha polypeptide antibodyFollicle stimulating hormone alpha chain antibodyFollicle stimulating hormone alpha subunit antibodyFollitropin alpha chain antibodyFSH alpha antibodyGlycoprotein hormones alpha chain antibodyGlycoprotein hormones alpha polypeptide 5 antibodyGlycoprotein hormones, alpha polypeptide antibodyLSH alpha antibodyLuteinizing hormone alpha chain antibodyLutropin alpha chain antibodyPituitary hormone antibodyThyroid stimulating hormone alpha chain antibodyThyrotropin alpha chain antibodyTSH alpha antibody
      see all

    References for Anti-Glycoprotein hormones (alpha 5) antibody [INN-hFSH-158] (ab11386)

    ab11386 has not yet been referenced specifically in any publications.

    Publishing research using ab11386 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab11386 Submit a Question for ab11386

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    Votes: Highest First

    Since each vial does 200 µg antibody, is the volume critical to the reaction? In other words, you supplied the antibodies at 1 mg/ml. Can't I use 200 µl antibody?

    Thank you for your reply and for your question.

    The lab has found that the volume is critical to the reaction, and theefficiency of the reaction will be significantly lower if more than 100 uL used. With 200 uL, I would be concerned that very li...

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    Inquiry: Hello, I am setting up an ELISA assay for free-alpha hCG. I have your coating antibody ab11387 and two detection antibodies ab11232 and ab11386. I want to biotinylate the detection antibodies so I can use streptavidin HRP and substrate to produce...

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    Thank you for contacting us with your question.

    We do have abiotinylation kit thatis suitable to use with these detection antibodies, ab102864-

    http://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html ...

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    Dear Charlotte Milnes, Thank you very much for your answer. I still need your help in the following points. What does "the alpha 5 epitope on the alpha subunit" means in your online data sheet? Does "the alpha 5" represent some specific region of ...

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    Thank you for your enquiry. I have gained confirmation of the and the list is cited below: Nicked human chorionic gonadotropin (hCGn) Carboxy terminal peptide of hCG (ctp hCG) Glycoprotein hormones (GPH) Human chorionic gonadotropin-beta (hCGb...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"