GnRH antibody (ab16216)

Product Name 

GnRH antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GnRH

Immunogen 

Synthetic peptide: pyroEHWSYGLRPG, corresponding to amino acids 22-31 of Mouse GnRH.
BLAST 'pyroEHWSYGLRPG' with or

Reacts with 

Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Tested applications 
(see key)

IHC-Fr

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at a concentration of 1 µg/ml. 4% paraformaldehyde or 2% acrolein is recommended as a fixative. Staining of GnRH in newborn mouse brain tissue using ab16216 results in neuronal staining.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Developmental Biology >> Reproduction >> Hormones
Signal Transduction >> Growth Factors/Hormones >> Hormones

Relevance 

Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.
Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1mg/ml BSA, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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