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- Secondary antibodies
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Product name
Anti-GnRH antibody
See all GnRH products (10) ...
Description
Mouse monoclonal to GnRH
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human, Chimpanzee, Rhesus monkey
Immunogen
Recombinant fragment: QHWSYGLRPG GKRDAENLID SFQEIVKEVG QLAETQRFEC TTHQPRSPLR DLKGALESL IEEETGQKKI, corresponding to amino acids 24-93 of Human GnRH (NP_000816) with a tag (MW of the tag alone is 26 KDa).
QHWSYGLRPGGKRDAENLIDSFQEIVKEVGQLAETQRFEC TTHQPRSPLR DLKGALESLIEEETGQKKI
Positive control
Recombinant tagged Human GnRH fragment.
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Developmental Biology >> Reproduction >> Hormones
Signal Transduction >> Growth Factors/Hormones >> Hormones
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Western blot - GnRH antibody (ab76560)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa.This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA: Use at an assay dependent dilution. Detection limit for recombinant tagged GnRH is approximately 30ng/ml if used as a capture antibody.
Target
Relevance
Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
Cellular localization
Secreted
Alternative names
- GNRH 1 antibody
- GNRH1 antibody
- Gonadotrophin releasing hormone 1 antibody
see all
Database links
- Entrez Gene: 2796 Human
- Omim: 152760 Human
- SwissProt: P01148 Human
Anti-GnRH antibody images:
Western blot - GnRH antibody (ab76560)
Anti-GnRH antibody (ab76560) at 1 µg/ml + Recombinant tagged human GnRH fragment at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 10 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 34 kDa.
References for Anti-GnRH antibody (ab76560)
ab76560 has not yet been referenced specifically in any publications.
Publishing research using ab76560? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"