GnRH antibody (ab8491)

Product Name 

GnRH antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GnRH

Immunogen 

Full length protein (Human) conjugated to BSA.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, RIA

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
This antibody is suitable for use on paraffin embedded tissues without enzymatic antigen retrieval
Advised working dilutions are 1:50-1:100.
Dilution buffer is 0.15 M PBS with 1% Ovalbumin and 0.09% sodium azide.
Optimal dilution should be tested by serial dilution.

Positive control 
(see definition)

Hypothalamus

Cellular localization 

Secreted

Research areas 

Developmental Biology >> Reproduction >> Hormones
Signal Transduction >> Growth Factors/Hormones >> Hormones

Relevance 

Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.
Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

Whole antiserum

Storage buffer 

Liquid antiserum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

LHRH is also known as Gonadotropin-releasing hormone (GNRH).

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