Goat F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L (DyLight® 550), pre-adsorbed (ab98581)
- Product nameGoat F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L (DyLight® 550), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesHuman
- Tested applicationsICC/IF, Flow Cyt, IHC-P more details
Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
- Storage instructionsStore at +4°C in the dark.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab98581 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 550.
- Clonality Polyclonal
- General notesDyLight® 550 replaces DyLight® 549.
- Research Areas
Our Abpromise guarantee covers the use of ab98581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: 1/50 - 1/500.|
|Flow Cyt||Flow Cyt: 1/50 - 1/200.|
|IHC-P||IHC-P: 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.|
Goat F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L (DyLight® 550), pre-adsorbed images
Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
References for Goat F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L (DyLight® 550), pre-adsorbed (ab98581)
ab98581 has not yet been referenced specifically in any publications.