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Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2, pre-adsorbed (ab98754)

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Overview

  • Product nameGoat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2, pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesMouse
  • SpecificityAntiserum has been cross adsorbed against human and rat immunosorbents.
  • Tested applicationsICC, IHC-P, ELISA, WB more details
  • Minimal
    cross-reactivity
    Human, Rat more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab98754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC ICC: Use at an assay dependent dilution.
IHC-P IHC-P: 1/200 - 1/2000.
ELISA ELISA: 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1,000-1/30,000 dilution.
WB WB: 1/1000 - 1/30000. Colorimetric: Use 1/1,000-1/10,000 dilution; Chemiluminescent: Use 1/1,000-1/30,000 dilution.

References for Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2, pre-adsorbed (ab98754)

ab98754 has not yet been referenced specifically in any publications.

Publishing research using ab98754 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab98754 Submit a Question for ab98754

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Inquiry: I am looking for an un-conjugated secondary antibody which is Fab-specific for mouse IgG. Your product ab98754 appears to be suitable however I would need it to be specific to both (Fab') and (Fab)2 mouse IgG. Can you confirm if this product is b...

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Thank you for your patience.

I confirmed with the lab and am happy to forward their confirmation that ab98754 will detect both the (Fab) and the (Fab’)2 fragments of mouse IgG.

Please note that ab98754 itself is also a (Fab')2 fr...

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We generally use the Vector Laboratories ABC kits for IHC. General protocol is as follows (minus wash steps) 1. Frozen sections (mouse spleen) are fixed in cold acetone for 15 min. 2. Endogenous peroxidase blocked with H2O2 in methanol. 3. Blocking s...

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Thank you for this information.

I have noted that this primary/secondary antibody combination has been used and reviewed by another customer. Their Abreview includes a detailed protocol which you may wish to review. This review is at:http://www...

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I have a question aboutab8978.
I found that it is mouse monoclonal and was tested in mouse sample on the data sheet.
I thinks that it is theoretically impossible because this antibody have to use anti-mouse IgGas secondary antibody.
So it c...

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Thank you for contacting us.

Indeed, a mouse-on mouse staining can be quite tricky; however, it is not impossible. The background will depend on the fixation method ( perfusion fixed tissue vs. normally fixed tissue), on the tissue itself ( eg....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"