Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (Biotin) (ab102185)
Overview
- Product nameGoat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (Biotin)See all IgG secondary antibodies ...
- Target speciesRat
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Tested applicationsIHC-P, ICC/IF, ELISA, WB more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab102185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/500 - 1/5000. |
| ICC/IF | ICC/IF: 1/200 - 1/1000. |
| ELISA | ELISA: 1/10000 - 1/200000. (Primary) |
| WB | WB: 1/10000 - 1/200000. Using chemoluminescence detection. |
References for Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (Biotin) (ab102185)
ab102185 has not yet been referenced specifically in any publications.
