Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (Biotin), pre-adsorbed (ab6275)

Overview

• Product nameGoat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (Biotin), pre-adsorbedSee all IgG secondary antibodies ...
• Target speciesRat
• SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
• Tested applicationsICC/IF, Dot Blot, WB, ELISA, IHC, Immunomicroscopy more details
• Minimal
  cross-reactivity
  Cow, Horse, Human
  To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
  more details
• Immunogen

  Rat IgG F(ab')2 fragment

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
• Concentration information loading...
• Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Secondary antibodies
  • anti-Rat
  • IgG
  • Biotin

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications