Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (DyLight® 488), pre-adsorbed (ab102222)
Overview
- Product nameGoat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (DyLight® 488), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesRat
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
- Tested applicationsICC, IHC-P, Flow Cyt more details
-
Minimal
cross-reactivity
Human, Mouse more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C in the dark.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab102222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: 1/50 - 1/500. |
| IHC-P | IHC-P: 1/50 - 1/500. |
| Flow Cyt | Flow Cyt: 1/50 - 1/200. |
References for Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - (Fab')2 (DyLight® 488), pre-adsorbed (ab102222)
ab102222 has not yet been referenced specifically in any publications.
