Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC) (ab5928)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Functional Studies, ICC/IF, Immunomicroscopy, Flow Cyt
Overview
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Product name
Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
Tested applications
Suitable for: Functional Studies, ICC/IF, Immunomicroscopy, Flow Cytmore details -
Immunogen
Mouse IgM mu chain
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Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG F(ab’)2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Functional Studies |
1/10000 - 1/50000.
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ICC/IF |
1/1000 - 1/5000.
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Immunomicroscopy |
Use at an assay dependent dilution.
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Flow Cyt |
1/500 - 1/2500.
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Notes |
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Functional Studies
1/10000 - 1/50000. |
ICC/IF
1/1000 - 1/5000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
1/500 - 1/2500. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab5928 has been referenced in 2 publications.
- Bataille A et al. In Vitro Differentiation of Human Skin-Derived Cells into Functional Sensory Neurons-Like. Cells 9:N/A (2020). PubMed: 32316463
- Ishikawa M et al. Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model. Invest Ophthalmol Vis Sci 55:8531-41 (2014). ICC/IF ; Rat . PubMed: 25406290