Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHC
Overview
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Product name
Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.. -
Tested applications
Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
Minimal
cross-reactivity
Cow, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Rabbit IgG F(ab’)2 fragment
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Dot blot |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ELISA |
1/100000.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
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Electron Microscopy |
Use at an assay dependent concentration.
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IHC |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ELISA
1/100000. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Electron Microscopy
Use at an assay dependent concentration. |
IHC
Use at an assay dependent concentration. |
Images
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IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (45)
ab6112 has been referenced in 45 publications.
- Liao Y et al. HSF1 inhibits microglia activation to attenuate neuroinflammation via regulating miR-214-3p and NFATc2 in Parkinson's disease. Folia Neuropathol 61:53-67 (2023). PubMed: 37114961
- Wang Y et al. Overexpression of ARHI increases the sensitivity of cervical cancer cells to paclitaxel through inducing apoptosis and autophagy. Drug Dev Res 83:142-149 (2022). PubMed: 34189759
- Li S et al. CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer. J Gastroenterol Hepatol 37:154-163 (2022). PubMed: 34734434
- Liu Y et al. Circular RNA ROCK1, a novel circRNA, suppresses osteosarcoma proliferation and migration via altering the miR-532-5p/PTEN axis. Exp Mol Med 54:1024-1037 (2022). PubMed: 35879346
- Serrano-López EM et al. Deciphering the Role and Signaling Pathways of PKCα in Luminal A Breast Cancer Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36430510