Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed (ab6267)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins. -
Tested applications
Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Human more details -
Immunogen
Rat IgG F(ab’)2 fragment
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Conjugation
Texas Red ®. Ex: 596nm, Em: 620nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.5 moles Texas Red per mole of Goat IgG F(ab’)2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | ||
ELISA | ||
Immunomicroscopy | ||
Flow Cyt |
Notes |
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IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (14)
ab6267 has been referenced in 14 publications.
- Qi JL et al. SQSTM1/p62 regulate breast cancer progression and metastasis by inducing cell cycle arrest and regulating immune cell infiltration. Genes Dis 9:1332-1344 (2022). PubMed: 35873020
- Shepherdson JL et al. Delineation of the 1q24.3 microdeletion syndrome provides further evidence for the potential role of non-coding RNAs in regulating the skeletal phenotype. Bone 142:115705 (2021). PubMed: 33141070
- Wang S et al. Relationship between long non-coding RNA PCAT-1 expression and gefitinib resistance in non-small-cell lung cancer cells. Respir Res 22:146 (2021). PubMed: 33980216
- Zheng H et al. Expression profiling of mitochondria-associated microRNAs during osteogenic differentiation of human MSCs. Bone 151:116058 (2021). PubMed: 34144232
- Xiao H et al. Metformin ameliorates bleomycin-induced pulmonary fibrosis in mice by suppressing IGF-1. Am J Transl Res 12:940-949 (2020). PubMed: 32269725