Goat Anti-Human IgA alpha chain (beta-galactosidase) (ab136778)

Overview

  • Product name
    Goat Anti-Human IgA alpha chain (beta-galactosidase)
    See all IgA secondary antibodies
  • Host species
    Goat
  • Target species
    Human
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, ICC, WBmore details
  • Immunogen

    Highly purified Human IgA immunoglobulin alpha heavy chain.

  • Conjugation
    beta-galactosidase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Constituents: 50% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab136778 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Preparation of substrate: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

ab136778 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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